The invention relates to a system (10) for determining a force applied to a cell or tissue culture (14) arranged in a culture chamber (12), comprising an elastic element (30) mounted in or suitable for mounting in said culture chamber (12), said elastic element (30) being adapted to be coupled with said cell or tissue culture (14) such that a force applied to said cell or tissue culture (14) leads to a deflection of said elastic element (30) against a restoring force thereof, and said elastic element comprising a magnetic element (34) arranged such as to be moved upon deflection of said elastic element (30), a magnetic field sensor (42) mounted or suitable for mounting outside said culture chamber (12), said magnetic field sensor (42), when mounted outside said culture chamber (12), being adapted to detect a change of magnetic field attributable to a corresponding movement of said magnetic element (34) upon deflection of said elastic element (30).

Apparatus and methods for culturing cells in a cell culture medium including magnetic beads in a fluid. A variable magnetic field can be applied to the magnetic beads to create shear forces on cells on the surface of the beads. In certain embodiments, a rotational force can also be applied to the magnetic beads and the magnetic force can counteract the rotational force.

We describe an improved conductive biocompatible scaffold for electroporation. The biocompatible scaffold comprises biocompatible material (e.g., collagen and/or other extracellular matrix) and incorporates a metal or polymer network or dispersion that conducts an electrical current through the scaffold. The material can adsorb or trap or bind nucleic acids (e.g., RNA or DNA), nanoparticles, proteins and/or small molecules; subsequently cells are placed in proximity to or in contact with the scaffold and an electrical current passed through the scaffold, thereby facilitating cellular entry of the nucleic acids, nanoparticles, proteins and/or small molecules, particularly into those cells that are in proximity of the scaffold.

A device and method for cultivating cells are provided, wherein a cell cultivating layer is formed on a surface of a substrate, and a temperature-responsive layer having a plurality of temperature-responsive polymer with magnetic objects is formed on a surface of the cell cultivating layer. When a controlling characteristic exerted on the temperature-responsive layer is varied, a plurality of cells can be adhered on the cell cultivating layer or detached therefrom. When the device is utilized, a temperature control step is operated to control environmental temperature at a first temperature for inducing temperature-responsive polymers becoming hydrophobic whereby the plurality of cells are adhered on the cell cultivating layer. In addition, the alternating magnetic field is utilized to rise temperature of the temperature-responsive polymers so that the plurality of cells can be kept being adhered on the cell cultivating layer at a second temperature lower than the first temperature.

The present invention discloses a circulating magnetoelectric-induction reaction system and application thereof. The system comprises an alternating induction voltage unit, an alternating induction magnetic field unit, a low-frequency power supply, and a feed liquid container. The alternating induction voltage unit is mainly composed of a closed iron core, a primary coil, a secondary coil, and an induction voltage cavity. The alternating induction magnetic field unit is mainly composed of a C-shaped iron core, a primary coil, and a magnetic field cavity. The low-frequency power supply is connected to the primary coils in the alternating induction voltage unit and the alternating induction magnetic field unit and provides excitation voltage for the primary coils. The secondary coil comprises an insulating pipeline, which serves as a feed liquid circulating pipeline, and has both ends exposed out of the induction voltage cavity, with one end as a feed inlet and the other as a discharge outlet. The feed liquid container communicates with the feed liquid circulating pipelines in the alternating induction voltage unit and the alternating induction magnetic field unit to form a feed liquid circulation loop. Through the application, continuous-flow processing can be achieved, electrochemical reaction and ionic polarization can be avoided, and production and processing can be conducted efficiently and rapidly in a large scale.

The present invention refers to a process, and to the related apparatus, for the in vitro preparation of a biomimetic tissue prostheses of the tympanic membrane from mesenchymal stem cells; such prostheses are used for repairing or reconstructing the injured tympanic membrane in patients needing it, for example as a consequence of various trauma or pathologies.

The present invention provides an apparatus for applying electrical stimulation to cells in a liquid culture medium without using electrodes immersed in the liquid culture medium. This apparatus is an electrical stimulation apparatus A for electrically stimulating cells in a liquid culture medium (2). A liquid culture medium vessel (3) includes: a ring-shaped recess (7) for holding the liquid culture medium (2); and a through hole (6) formed within the ring-shaped recess (7). A magnetic core (1) is made of a magnetic material and disposed such that a portion thereof is inserted through the through hole (6) of the liquid culture medium vessel (3). An excitation coil (5) is wound around the magnetic core (1). A coil power supply (8) supplies a varying current to the excitation coil (5).

Disclosed is an in vitro exposure system that may radiate a uniform field having a constant wavefront to an experimental cell container and expose each cell container to a same electromagnetic field.

There is a need for fabrication of a three-dimensional structure for forming a regenerated tissue/regenerated organ having a desired size, shape, or structure by adjusting culture conditions with high precision and without causing contamination or damage to cells. Provided are: a device for acquiring a fabrication condition of a three-dimensional structure to be fabricated on the basis of externally inputted information relating to the three-dimensional structure, generating a magnetic field in a culture container for cells on the basis of the acquired fabrication condition, supplying magnetized cells to the culture container in which the magnetic field is generated, and culturing the supplied cells; and a method using the device.

A method of producing a cell structure includes applying a magnetic field, in a container (40), to a plurality of culture carriers to arrange the culture carriers. The culture carriers include at least one of a carrier (10) and a cell holding carrier (30), the carrier (10) having a magnetic portion (12), formed only in a part of the carrier (10), and a cell holder (11). The method also includes culturing cells (20) held on the cell holder (11) while maintaining the culture carriers in the arranged state.