A bioreactor and method of forming complex three-dimensional tissue constructs in a single culture chamber. The bioreactor and methods may be used to form multi-phasic tissue constructs having tissue formed from multiple cell types in a single culture chamber. The bioreactor includes at least one translation mechanism to facilitate translation of one or more tissue constructs without direct user intervention, thereby providing a closed, sterile environment for complex tissue fabrication. The bioreactor may be used as a stand-alone device or as part of a large-scale system including many bioreactors. The large-scale system may include a perfusion system to monitor and regulate the tissue culture environment.

The present disclosure provides a microfluidic device for simulating a blood-brain barrier and a blood-brain barrier simulation system including the same, and the microfluidic device includes: a first channel; a second channel which is adjacently connected to the first channel through one or more microholes and configured to culture neural stem cells; and a chamber which is connected to both ends of the first channel and contains a culture medium.

A cell culture chip has a stack structure formed by sequentially stacking: a first electrode provided on a main surface of a first board; a first partition wall layer including a first main flow path, and a first inlet flow path and a first outlet flow path connected to the first main flow path; a planar mesh structure sheet used as a scaffolding material for cells; a second partition wall layer including a second main flow path, and a second inlet flow path and a second outlet flow path connected to the second main flow path; and a second electrode provided on a main surface of a second board, in which the planar mesh structure sheet is sandwiched between the first partition wall layer and the second partition wall layer, and, among aperture ratios of a surface of the planar mesh structure sheet facing the first partition wall layer, an aperture ratio of a portion facing the first main flow path is greater than an aperture ratios of portions facing the first inlet flow path and the first outlet flow path, and, among aperture ratios of a surface of the planar mesh structure sheet facing the second partition wall layer, an aperture ratio of a portion facing the second main flow path is greater than an aperture ratios of portions facing the second inlet flow path and the second outlet flow path.

In an embodiment, the present disclosure pertains to an organ-chip model having a plurality of cell culture chambers connected through arrays of microfluidic channels. In some embodiments, each cell culture chamber of the plurality of cell culture chambers include an inlet and an outlet. In some embodiments, the inlet is configured to receive at least one of a cell, cell media, or a cell stimulant. In some embodiments, at least one outlet is configured to collect effluent. In some embodiments, the organ-chip model can include, without limitation, an organ-chip model of amnion membrane, an organ-chip model of a feto-maternal interface (fetal membrane-decidua parietalis), an organ-chip model of a feto-maternal interface (placenta-decidua interface), an organ-chip model of a cervix, and combinations thereof. In some embodiments, the organ-chip model is an interconnected organ-chip model having a combination of one or more organ-chip models with interconnected cell culture chambers.

An in situ cell bioreactor and delivery system is provided. The system is composed of toroidal-spiral particles, which encapsulate cells therein and can further provide one or more active agents. Methods for using the system in cell-based therapies are also provided.

The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.

A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

Disclosed are systems and methods for culturing systemic bioengineered tumor models, including osteosarcoma cells or breast adenocarcinoma cells derived from patients. The model includes the tumor cells on host tissues such as bone, liver, lung, and heart tissue, wherein the bone, liver, lung, and heart tissue are separated by endothelial barriers. Beneath the endothelial barriers the tissues are connected via circulation containing secreted factors and cells (e.g., tumor/immune cells).

Methods and systems are provided for transfecting cells using real-time, continuous transfection of cells. In some aspects, the methods can be applied for the continuous production of non-viral vector nucleic acid complexes. The systems and methods include a passive mixing fluidic module with at least two inlets, a plurality of mixing elements, and an outlet to provide a continuous flow of transfection complexes to a cell reactor. The transfection agent and nucleic acid are passively mixed and then provided to cells in a continuous flow of cell medium. In some aspects, the flow of cell medium perfusing through the cell reactor recirculates. The system and the methods of the present disclosure provide for highly reproducible and scalable transfection with a low coefficient of variation.

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.