The presently disclosed subject matter provides a microfluidic device that can simulate capillary blood flow on a fetal side of the device and pooled blood on a maternal side of the device (i.e., intervillous space). The microfluidic device can reconstitute the maternal-fetal interface, can expand the capabilities of cell culture models, and can provide an alternative to current maternal-fetal transfer models.

A method to provide a microfluidic flow comprising a central flow and at least one outer flow, such that the central flow includes a first material and the at least one outer flow comprises a second material. One of the first material and the second material has cells and the other of the first material and the second material has solid particles. The method involves injection of a first suspension including the first material through a central inlet with a flow rate Q2 and injection of a second suspension comprising the second material through a pair of side inlets with a flow rate Q2, whereby the ratio of the flow rate Q2 over the flow rate Q1 is at least 4. A device provides such microfluidic flow and a method is provided to alter biological cells.

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

A device includes a first unit for skeletal muscle tissue formation; a second unit for motor neuron culture; a third unit for causing the first and second units to communicate with each other; and a pillar serving as a scaffold for skeletal muscle tissue formation. The first unit includes a first base material and a first culture tank formed in the first base material. The second unit includes a second base material and a second culture tank formed in the second base material. The third unit includes a third base material and an axon channel formed in the third base material, through which a bundle of axons passes. One end of the third unit is connectable to the second unit and cause the axon channel and the second culture tank to communicate with each other. A first opening part is formed to the other end of the third unit.

In one aspect, provided is a composition (biomimetic composition) that includes a biomimetic in vitro model of an arteriolar vessel comprising: at least one of 1) human smooth muscle cells and 2) human pulmonary endothelial cells; wherein the vessel recapitulates one or more of the overall tubular geometry, morphometrics, extracellular matrix constituents, cellular morphology, cellular alignment, and functional heterotypic connections between the human smooth muscle cells and/or the human endothelial cells as compared to an in vivo arteriolar vessel. A microfluidics-based model platform of the pulmonary circulation is provided. Methods of use include measuring flow in biomimetic vessels, and to determine the resistance of these biomimetic vessels in the setting of a variety of experimental conditions that recapitulate the pathobiology of pulmonary hypertension.

An object of the present invention is to provide an artificial tissue perfusion device capable of analyzing the interaction between a vascular cell layer and a parenchymal cell layer with high accuracy. An artificial tissue perfusion device includes a co-culture system (C) in which a plurality of types of cell are cultured. The co-culture system has a tubular well part (10) having a culture space (11) inside; a base material (20) having a perfusion flow path (26) which extends in a predetermined direction and is perfused with a medium, and a holding part (23) which opens to the perfusion flow path and holds the well part attachably and detachably; and a gel membrane (30) having a form of a porous membrane and disposed at an end portion of the well part facing the perfusion flow path in a case where the well part is held by the holding part. A tissue-based cell is cultured on a surface side of the gel membrane facing the culture space, and a luminal cell is cultured on a surface side of the gel membrane facing the perfusion flow path.

The present disclosure relates, at least in part, to a closed and semi-automated system for the isolation of naive T cells, their expansion, and/or final harvest. The disclosure also relates to using those isolated cells in a large batch format for compiling stocks of stimulated CD45A+ T cells and/or using the stimulated CD45A+ T cells for therapeutic purposes.

The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood, and the automated generation of immunotherapeutic products from those dendritic cells, all within a closed system. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.

Disclosed is a process for growing adherent cells in a containment box of a multi-parallel bioreactor, including: seeding the adherent cells on a carrier held in a culture dish; transferring the adherent cells on the carrier to a containment box of the multi-parallel bioreactor; and growing the adherent cells at a containment box while agitating the media at an impeller speed between 200 rpm to a 1200 rpm.