Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 .Math.m or smaller, and 0 < B/A ≤ 0.5 is satisfied in a case where an average hole diameter of the first filter membrane is A and an average hole diameter of the second filter membrane is B; or an average hole diameter of the first filter membrane is 20 .Math.m or smaller, and the second filter membrane is an ultrafiltration membrane.

An improved fluid filtration system is described. The system uses improved sensor assemblies to avoid the problem of biomass build up and reduce the holdup volume of liquid outside of the process vessel. It significantly enhances the performance, robustness and consistency of the filtration.

In some variations, a UV-sterilizable bioreactor system comprises: a chamber configured to carry out a reaction; a component configured for introducing a gas into the chamber; and one or more UV light sources configured to expose ultraviolet light to surfaces within the chamber. Some variations provide a method of cleaning and sterilizing a bioreactor, comprising: providing a bioreactor system with a chamber to carry out a reaction, a component for introducing a gas into the chamber, and one or more UV light sources to expose ultraviolet light to chamber surfaces; cleaning the chamber; and exposing the chamber to UV radiation to sterilize the chamber. The disclosed technology is a fundamental advance in the field of sparged bioreactors, for many types of commercial fermentations. The bioreactor may incorporate materials that are expressly incompatible with steam sterilization, which allows for the use of lower-cost materials, among many other benefits from UV sterilization.

A culture device includes a culture vessel that contains a culture solution for culturing cells, a gas supply device that supplies a gas to the culture vessel, and a humidification device that humidifies the gas flowing from the gas supply device to the culture vessel. The humidification device includes a hollow fiber membrane filter that includes a hollow fiber membrane through which the gas from the gas supply device passes and a casing that accommodates the hollow fiber membrane, a water supply device that fills the casing of the hollow fiber membrane filter with water, and a first heater that heats the hollow fiber membrane filter.

The present invention relates to a device (100) for monitoring the development of a biological material, said device in the orientation intended for use comprising: a housing (2) having an extension in a longitudinal direction (X) and an extension in a transversal direction (Y), said housing comprises: two or more culture dish compartments (4), each adapted to accommodate a culture dish (6) comprising a biological material (8) to be monitored, each said compartment being separated from each of said other compartments, said two or more compartments being arranged along the longitudinal direction; each said culture dish compartment (4) comprising a lid (10) adapted to be able to be shifted between an open configuration in which access to the interior (12) of said culture dish compartment is provided, and a closed configuration sealing off the interior of said culture dish compartment from its surroundings; wherein each said culture dish compartment comprises an inlet (14) for supplying gas to and an outlet (16) for removing gas from said culture dish compartment; wherein each said culture dish compartment comprising heating means (18) for heating the interior of said culture dish compartment; wherein said housing comprises one or more cameras (20) for capturing images of a biological material in a culture dish.

A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.

Embodiments of the present disclosure include methods and systems for detecting the presence of endotoxins in filters. For example, embodiments of the present disclosure are directed to methods and systems for improving in-process controls by detecting undesirable quantities of endotoxins in filters containing naturally-sourced materials prior to using those filters in the production of formulated drug substances.

The present invention involves a fill needle system for aseptically dispensing a pharmaceutical fluid in an aseptic chamber comprises a fill needle tubing in fluid communication with a pharmaceutical fluid source via flexible tubing and extending through a fill needle hub; a fill needle dispensing tip disposed at a dispensing end of the fill needle tubing; a fill needle sheath shaped and arranged to removably mate with and seal aseptically to the fill needle hub to form an aseptically sealed volume enclosing the dispensing tip; and a fluid pressure pulse induction system disposed and configured to compress the flexible tubing in order to dislodge droplets of pharmaceutical fluid retained on the dispensing tip after halting dispensing of the pharmaceutical fluid. An associated method of dispensing pharmaceutical fluid comprises operating the fluid pressure pulse induction system to dislodge the droplets. The system may comprise a controller for automatically controlling the dispensing and droplet dislodging.

A resource treatment system for urine and feces separation and recovery in urine diversion dehydration toilets, includes a urine-faeces division toilet, a urine and gray water treatment system, and a fermentation and biodegradation fecal system. The urine-faeces division toilet is configured to separate and recover urine and feces discharged by users. The urine and gray water treatment system includes an adjusting pool, a microalgae culture device and a metal-based electrogenerated dynamic membrane. The adjusting pool is configured to receive the urine in the urine-faeces division toilet and domestic sewage, and adjust a urine-to-domestic sewage ratio. The metal-based electrogenerated dynamic membrane includes a metal microfiltration membrane, a stainless-steel mesh and a power supply. The fermentation and biodegradation fecal system includes a collection and adjusting device, a fermentation bed and a biodegradation chamber.

A probe assembly for inserting a probe into a vessel or tubing includes a probe sheath having a proximal end and a distal end and being configured for operative coupling to a vessel or tubing, the probe sheath being configured to receive a probe and to permit movement of the probe towards the distal end of the probe sheath, and a locking mechanism configured to restrain longitudinal movement of the probe with respect to the sheath in a locked state. The locking mechanism may be unlocked to allow for movement of the probe with respect to the sheath.