A system to reduce surface bubbles from cell culture media using ultrasonic pulses is disclosed. The system includes a plurality of ultrasonic transducers, a photodetector, a camera, a control circuit, and a cell culture container that includes a cell culture media. The control circuit is communicatively coupled to the plurality of ultrasonic transducers, the temperature sensor, and the camera. The photodetector is used to determine a presence of a surface bubble on the cell culture media. The camera is used to determine a location of the surface bubble when the presence of the surface bubble is determined. The plurality of ultrasonic transducers is used to position an ultrasonic pulse at the location of the surface bubble and thereby reduce the presence of the surface bubble on the cell culture media when the location of the surface bubble is determined.

A vessel configured to contain a liquid is provided. The vessel includes a collapsible bad, a reusable support structure, a sensor, and a controller. The collapsible bag is operative to contain the liquid. The reusable support structure is operative to support the collapsible bag. The sensor is operative to measure a parameter within the collapsible bag. The controller is in electronic communication with the sensor and operative to introduce an antifoaming agent into the collapsible bag based at least in part on a signal received from the sensor.

A vessel configured to contain a liquid is provided. The vessel includes a collapsible bad, a reusable support structure, a sensor, and a controller. The collapsible bag is operative to contain the liquid. The reusable support structure is operative to support the collapsible bag. The sensor is operative to measure a parameter within the collapsible bag. The controller is in electronic communication with the sensor and operative to introduce an antifoaming agent into the collapsible bag based at least in part on a signal received from the sensor.

A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.

The invention relates to a biogas plant fermenter tank (1) comprising a substantially liquid substrate (6) accommodated in the tank inner chamber (5) and at least one stirring device (8) arranged in the tank inner chamber (5). According to the invention, an injection device (9) having at least one delivery pump (10) which extracts the injection liquid (12), and at least one injection nozzle element (11) which is fluidically connected to the at least one delivery pump (10), is provided. The injection liquid (12) can be injected into the fermenter tank (1) by means of said injection device in such a way that the injection liquid impinges as a liquid jet (13) from above onto the surface of a floating layer (7) of the substrate (6) and wets the latter.

The present disclosure provides a method of preparing a high density culture of bacteria. The method provides a bioreactor comprising a fermentation tank, a perfusion system, a medium, and a cell separator; preparing an inoculum comprising a bacteria consortia with at least two bacteria species; inoculating the fermentation tank with the inoculum to form a fermentation culture; and growing the bacteria consortia until it has reached a target density of at least about 10.sup.12 CFU/mL.

The aspects of the disclosed embodiments generally relate to an apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments also generally relate to a method of using such apparatus which allows for the controlled addition of antifoam to the foam present in the headspace of a disposable single-use bioreactor in a reliable manner. The aspects of the disclosed embodiments generally relate to antifoam systems, methods and apparatus, and more particularly, to an antifoam device operably connected to a single use biobag.

The present invention relates to the field of fermentation. In particular, the present invention relates to a method and apparatus for production of products by use of living cells or active components derived from such cells using a bioreactor having a built in gas distributor and a specific system/device for handling foam formed in the process. The invention also relates to a bioreactor comprising: a reaction chamber having a fermentation zone and a foam settling zone, wherein the reaction chamber is arranged such that the foam settling zone is spaced from or physically separated from the fermentation zone so as to reduce the effect of activity in the fermentation zone on foam in the settling zone. The separation of the fermentation zone from the activity of the fermentation zone encourages settling of the foam. The invention also provides a method of forming a bioreactor comprising: overlapping two flat, flexible sheets of material; and causing said two sheets to adhere to each other in selected areas so as to define a reaction chamber. This is a particularly cost-effective way to produce a single use, disposable reactor.

A system for enabling a wireless single use sensing sub-system by optimizing electrical power is presented. The system includes the sensing sub-system configured to be employed in a bio process environment. Further, the sensing sub-system includes a sensing unit configured to measure at least one parameter of the bio process environment. Also, the sensing sub-system includes a power source electrically coupled to the sensing unit and configured to transmit an electrical power to the sensing unit. Furthermore, the sensing sub-system includes a switch configured to electrically couple or decouple the sensing unit from the power source. In addition, the system includes a control sub-system including a first controller configured to determine at least one power control parameter based on a user-input data and a sensing sub-system data, and optimize consumption of the electrical power in the sensing sub-system, based on the power control parameter.

Provided is a device which is capable of ongoing measurement of variable foaming tendencies in a fluid system and provide a signal to regulate the feed rate of defoamer accordingly to maintain foaming at an acceptable target level. The regulation of feed can be accomplished automatically or manually.