Methods and systems for controlling microorganism culture conditions are disclosed. The system may comprise a sample vessel and plumbing free metal parts, and use a modular cap design to adapt to a multitude of analytical probes, and possess a square profile with non-reflective surfaces. The system may also include stirrer; a lighting control module, a lighting element may produce stable light intensity range; a temperature control module that may be located at the bottom of the sample vessel; a pH control; a gas sparging control module; a dilution control module; a liquid storage system; an electronic modularity; a weighing module; and a scheduling system. In many embodiments of the system and method, the pH control may hold the pH within 0.1 of a target pH, the weighing module may monitor volume, and the scheduling system may allow the creation of user defined events and set points.

A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.

A system for electronically and optically monitoring biological samples, the system including: a multi-well plate having a plurality of wells configured to receive a plurality of biological samples, each of the wells having a set of electrodes and a transparent window on a bottom surface of the well that is free of electrodes; an illumination module configured to illuminate the wells; a cradle configured to receive the multi-well plate, the cradle having an opening on the bottom that exposes the transparent windows of the wells; and an optical imaging module movable across different wells of a same multi-well plate to capture images through the windows.

Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.

Described herein are bioreactors and systems comprising the same (e.g., a bioreactor assembly). Also described herein are methods of using the bioreactors and systems.

A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.

Systems and methods are provided for processing carbon dioxide-containing gas based on biomass. Such processing may include collection from a point source or atmosphere, drying, treatment, and compression. The compressed gas may be fed into an algae reactor that maintains optimal conditions for algae growth. The algae may be allowed to grow until a predetermined density associated with harvesting is reached. The harvested algae may be processed and packaged. The process may be monitored by sensors and controlled by a computing device in communication with the devices associated with each stage of processing.

A method of artificial-intelligence-based robotic vitrification includes placing at least one oocyte in a buffer or CPA solution. The method includes processing an image object produced by an imaging system, using an AI/ML system, to determine a location of a retrievable target oocyte. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target oocyte. The method includes confirming the robotic pipettor's location at the target physical orientation, using the AI/ML system. The method includes instructing the robotic pipettor to initiate contact with the retrievable target oocyte and apply negative pressure to secure the oocyte to the robotic pipettor. The method includes using a robotic microtool holder to lower a vitrification assembly or cryo-device of any other make into a buffer or CPA solution dish to a position in proximity to the target physical orientation.

A contactless selection device, a light triggering structure thereof, and a biological particle selection apparatus are provided. The light triggering structure includes a first substrate, a first electrode layer formed on the first substrate, a photodiode layer formed on the first electrode layer, and an insulating layer that covers the photodiode layer. The photodiode layer has a thickness within a range from 1 m to 3 m, and includes a first doped layer, an I-type layer, and a second doped layer, which are sequentially stacked from the first electrode layer. The second doped layer includes a plurality of triggering pads spaced apart from each other. Each of the triggering pads has a width within a range from 3 m to 7 m, and a distance between any two of the triggering pads adjacent to each other is less than or equal to 2 m.

The present invention relates to a method of determining the concentration of intact microorganisms in a sample comprising optionally diluting an aliquot of the sample to provide a diluted aliquot at a dilution value; contacting at least a portion of an aliquot or of a diluted aliquot of the sample with first and second stains capable of binding to DNA, wherein the first stain is a fluorescent stain, is cell-permeable, and has a first emission wavelength, and the second stain is cell-impermeable, and is capable of acting as an acceptor molecule in a FRET pair with the first stain acting as a donor molecule; imaging the aliquot-stain mixture at the first emission wavelength and determining an image analysis value for the number of objects corresponding to intact microorganisms in the imaged mixture; and comparing the image analysis value for said aliquot to a pre-determined calibration curve, as well as an apparatus, a consumable and a kit therefor.