Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

There is provided a method of controlling operation of a fed batch process in a bioreactor vessel, comprising transitioning from a batch phase to a production phase in dependence on a relationship between an oxygen supply parameter O and a dissolved oxygen value DO.

An anaerobic digestion device based on self-sustained air flotation includes an anaerobic digestion tank unit, a self-sustained air flotation screening unit and a biogas measurement and collection unit. The self-sustained air flotation screening unit includes an air flotation screening part, a material sedimentation part, a reflux part and a three-phase separation part connected sequentially from bottom to top. A digested material in the anaerobic digestion tank unit is pumped into the air flotation screening part, overflows into the material sedimentation part, and then is raised to the reflux part. Gas passing through the three-phase separation part and gas produced in the anaerobic digestion tank unit enter the biogas measurement and collection unit to be measured and collected.

A system includes a bioreactor having an inlet and an outlet, a cell-media recirculation loop between the outlet of the bioreactor and the inlet of the bioreactor, and a mature cell separation unit positioned along the cell-media recirculation loop wherein the mature cell separation unit is configured for performing separation of mature cells from immature cells and direction of the immature cells back to the inlet of the bioreactor. The system may further include a storage unit for storing the mature cells. The mature cell separation unit may be further configured to direct the mature cells to a mature cell storage unit.

The present application focuses on systems and methods that utilize one or more carbon dioxide (CO.sub.2) sorbent substrates and a swing cycle, e.g., a moisture swing cycle, to increase the partial pressure of the CO.sub.2 in a gaseous feedstock, which is delivered through a membrane to a bioreactor, such as a membrane carbonation photobioreactor. Such systems and processes offer an effective means for concentrating and capturing CO.sub.2 obtained from air and delivering the concentrated CO.sub.2 to a photobioreactor through a membrane.

The invention relates to systems and methods for effective production and use of microorganisms and/or the fermentation broth in which they are produced. Advantageously, the system is cost-effective, scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

An automated apparatus for preparing a liquid bioprocess solution includes at least one mixing chamber having a lower port and an upper port for fluid to enter the at least one mixing chamber, an array of tubing for fluid flow within the system, a plurality of valves provided within the tubing, and a mixing controller configured to cause the automated apparatus to perform a series of sequential mixing steps causing the preparation of the liquid bioprocess solution from a dry ingredient. The series of sequential mixing steps include opening a first valve associated with the lower port to provide fluid to the at least one mixing chamber through the lower port, and after a predetermined amount of elapsed time, closing the first valve and opening a second valve associated with the upper port to provide fluid to the at least one mixing chamber through the upper port.

The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000L.

A fermentation unit and a method of carrying out fermentation are described. The fermentation unit can be transported from one facility to another to carry out a fermentation process. The fermentation unit and method allow for the fermentation of a C1-containing substrate from a source at a particular facility to produce products such as alcohols and acids. Examples of the source of the C1-containing substrate include without limitation steel manufacturing processes, coal and biomass gasification processes, coke manufacturing processes, etc. The fermentation unit can be housed within a container having a volume of less than about 6 m.sup.3.

A bioreactor system for cultivating microalgae is described. The bioreactor system includes a bioreactor. The bioreactor includes one or more holes. One or more light sources are implanted into each of the one or more holes. A culture media comprising a carbon source is located inside of the bioreactor. A microalgae comprising a photoreceptor sensitive to a region of a visible spectrum is located in the culture media. Each of the one or more light sources produce an irradiance of light including the region of the visible spectrum in a sufficient intensity to transduce the photoreceptor of the microalgae.