The present invention relates to a device and processes for producing liquid media for cell cultures, whereby the liquid media are produced automatically by dissolving ingredients in water. The present invention also relates to a device for producing media used in cell cultures or a substance produced by cell cultures using a bioreactor process.

The present application relates to biotechnological fluid treating, such as biopharmaceutical liquids in order to obtain products such as monoclonal antibodies, vaccines or recombinant proteins, and particularly to a system for treating a biotechnical fluid, having a bioprocess machine and at least one bioprocess machine helper, configured to connect to each other and each comprising a controller, in turn comprising a machine-to-machine communication tool configured for connecting to a network.

Clean-in-place methods applicable to improve production of fermentation operations are disclosed herein to monitor, report, and control the cleaning reagents applied, residual components thereof, and clean-in-place conditions within levels that are compatible with fermentation processes, without limiting the effectiveness of the clean-in-place protocol with respect to cleaning or sanitizing the fermentation system.

The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.

Processes, as well as associated systems and computer program (software) products, are disclosed for the biological conversion of CO into desired end products such as ethanol. The control methodologies used for these processes can advantageously result in a reduced time required for a batch operation or other initial operating period, prior to achieving a continuous operation, which may be demarcated either by the addition of fresh culture medium at a defined flow rate or by another process initiation target. The control methodologies may alternatively, or in combination, improve a process performance parameter, such as productivity of the desired end product or bacterial growth rate, during this batch operation or other initial operating period.

Methods and systems for measuring viability and function of islet cells or stem cell-derived beta cells in an implantable device featuring setting the temperature of the cells in the implantable device to a low temperature to reduce metabolic levels of the cells and reduce oxygen requirements of the cells, and measuring oxygen consumption rates. An oxygen sensor at the inlet of the implantable device and an oxygen sensor at the outlet of the implantable device are used to calculate oxygen consumption rates of the cells, which in turn are indicative of viability. The reduction in temperature can also be used for loading cells into the implantable devices to help reduce ischemic and/or physical injury. The present invention may be used with other cell types, e.g. hepatocytes, heart cells, muscle cells, etc.

A method for the anaerobic cultivation of microorganisms includes providing an aqueous solution having a pH value of 4.5 to 7.5 in a container, adding a substrate in a first substrate dosage to the aqueous solution, adding further elements to the aqueous solution, adding an inoculant with microorganisms to the aqueous solution, hermetically sealing the container, varying a temperature of the initial product or the intermediate products in a range from 40 to 80 degrees Celsius, taking a sample and determining a first concentration of organic substance in the sample, taking another sample and determining another concentration of organic substance in the further sample after the expiration of the first waiting time, if the concentration of organic substance is smaller than 10 percent of the first concentration of organic substance, adding substrate in another substrate dosage, repeating the above until a sufficient amount of biomass is present in the container.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

An animal-derived bacteria accelerated culture device includes: a bacteria accelerated incubator; support fixed feet installed on a lower end of the bacteria accelerated incubator; limit fixed frames installed on an end of the bacteria accelerated incubator facing away from the support fixed feet, each the limit fixed frame being provided with a limit fixed groove and a first fixed pin hole; a detection frame installed on an upper end of the bacteria accelerated incubator. By controlling working detection heads to detect bacteria inside the bacteria accelerated incubator, detection results are transmitted to a processing work case through detection positioning brackets for analysis and processing, and data is displayed by a data display device, the staff can adjust parameters such as temperature, potential of hydrogen (pH), oxygen, nutrients, etc. of the bacteria accelerated incubator through displayed values, thus improving the work quality and efficiency.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.