A fermentation unit and a method of carrying out fermentation are described. The fermentation unit can be transported from one facility to another to carry out a fermentation process. The fermentation unit and method allow for the fermentation of a C1-containing substrate from a source at a particular facility to produce products such as alcohols and acids. Examples of the source of the C1-containing substrate include without limitation steel manufacturing processes, coal and biomass gasification processes, coke manufacturing processes, etc. The fermentation unit can be housed within a container having a volume of less than about 6 m.sup.3.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

The present application provides a process for sequestering carbon dioxide to produce a biomass containing reaction product, the process comprising the steps of: I. contacting a raw carbon dioxide-containing feedstock with an absorption or dissolution medium to form a reagent stream comprising dissolved or absorbed inorganic carbon at least in the form of HCO.sub.3.sup.- and CO.sub.3.sup.2- wherein the HCO.sub.3.sup.- :CO.sub.3.sup.2- molar ratio in the reagent stream is at least about 0.8; II. contacting at least a portion of the reagent stream with a microbial broth in a bioreactor to produce a biomass-containing reaction product; III. separating the biomass-containing reaction product into a biomass product and a liquid stream; and IV. recycling at least a portion of the liquid stream to step i. of the process for use as, or as part of, the absorption or dissolution medium,wherein the pH of the absorption or dissolution medium is controlled to maintain the HCO.sub.3.sup.- :CO.sub.3.sup.2- molar ratio in the reagent stream at least about 0.8.

A method includes directing fluid flow from a bioreactor to an external path of a re-circulation loop, separating cells from the medium and returning the cells to the bioreactor thereby leaving spent medium, depleting the spent medium of biological waste products, replenishing the spent medium with beneficial growth factors to thereby provide replenished medium, and directing the replenished medium back to the bioreactor to thereby complete the re-circulation loop such that recycling of the medium occurs. The replenishing of the spent medium with beneficial growth factors may occur within a medium collection reservoir. The method may further include monitoring the spent medium within the medium collection reservoir including temperature of the spent medium within the medium collection reservoir and pH of the spent medium within the medium collection reservoir.

The present disclosure provides cartridges, pump assemblies, and other systems, methods, and apparatuses for manufacture of a cell therapy. A rigid cartridge includes rigid walls defining a rigid housing. An interior surface of a first rigid wall includes a first mating mechanism to engage a second mating mechanism of a docking device to removably coupled with the rigid cartridge. A rigid wall includes a planar upper surface including an aperture. The aperture is configured to receive and fixedly engage a corresponding connector.

A bioreactor includes a plurality of spargers and a plurality of vertical circulation loops. A first vertical circulation loop of the plurality of vertical circulation loops includes a first sparging region and a first return region. The first vertical circulation loop is in liquid communication with one or more other loops of the plurality of vertical circulation loops. The first vertical circulation loop is characterized by an individual loop mass transfer coefficient. A controller is coupled to the plurality of spargers and configured to control the plurality of spargers together such that a cumulative mass transfer coefficient of the plurality of vertical circulation loops is within a threshold of the individual loop mass transfer coefficient associated with the first vertical circulation loop.

An airlift bioreactor includes a body, a first sparging region, a second sparging region, a first sparger configured to sparge a first gas within the first sparging region, a second sparger configured to sparge a second gas within sparging region, and a controller coupled to the first sparger and the second sparger. The controller is configured to control a first flow rate associated with the first sparger and a second flow rate associated with the second sparger.

The present application relates to biotechnological fluid treating, such as biopharmaceutical liquids in order to obtain products such as monoclonal antibodies, vaccines or recombinant proteins, and particularly to a system for treating a biotechnical fluid, having a bioprocess machine and at least one bioprocess machine helper, configured to connect to each other and each comprising a controller, in turn comprising a machine-to-machine communication tool configured for connecting to a network

A system and a method for treating biomass with a gas includes at least one conduit having at least one biomass inlet and at least one biomass outlet, at least one gas inlet and at least one gas outlet. The system further includes a transport unit configured to move the biomass through the conduit from the at least one biomass inlet to the at least one biomass outlet thereby defining a biomass transport direction. The system is configured such that gas flowing from the at least one gas inlet to the at least one gas outlet crosses the biomass transport direction.

A bioreactor includes a rigid-walled vessel for containing a biological medium, the vessel having a lid. The bioreactor further includes a probe passing through an aperture in the lid and having a sensing end inside the vessel and a remote end outside the vessel. The sensing end has plural electrodes for immersion in the biological medium, and the remote end is configured for coupling to external devices and transmission thereto of electrical or electromagnetic measurements made by the electrodes. One of the lid and the probe has one or more resiliently deformable mechanisms and the other of the lid and the probe has one or more respective complementary surfaces. The resiliently deformable mechanisms and the complementary surfaces are configured such that, on insertion of the probe through the aperture to assemble the probe to the lid, the one or more resiliently deformable mechanisms are first deformed on sliding against the one or more complementary surfaces and then resile when the probe reaches a predetermined insertion position relative to the lid to secure the probe to the lid. One of the lid and the probe carries a sealing element which seals the probe to the lid when the probe is secured at the predetermined position.