The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

The present disclosure provides a bioreactor, which includes a tank configured to contain a mixture of animal cells and a liquid; and a first ventilation device arranged outside the tank and including a first gas distributor, the first gas distributor being configured to transmit a gas to the liquid separated from the animal cells. The bioreactor further includes a dialysis component, which is arranged outside the tank and comprises a dialysis filter. The bioreactor further includes a second ventilation device arranged in the tank and configured to transmit the gas to the mixture in the tank.

A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

The invention relates to a method for aerobic and anaerobic cultivation of microorganisms. The invention also relates to a method for producing a preparation for cleaning contaminated liquids and surfaces. Likewise, the invention relates to a method for cleaning contaminated liquids and surfaces.

Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.

The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACO.sub.R-M-.sub.ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO.sub.R-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.t i) and a current pH value (pH.sub.Bi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACO.sub.B1-tir PACO.sub.Bi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.ti) measured in the bioreactor from a predicted CO2 off gas rate (ACO.sub.B1-EXP-ti, ACO.sub.B2-E xp-.sub.t i). a difference between the computed PACO value (PACO.sub.Bi-ti, PACO.sub.B2-ti) and a respective reference PACO value (PACO.sub.R-ti) in the PACO-reference profile (116).

Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

A bioproduction mixer control system includes a mixing compartment having a biological fluid and a mixing element; a sensor in communication with the mixing compartment, the sensor configured to detect an environmental condition within the mixing compartment; and an integrated control unit. The integrated control unit includes a memory for storing the environmental condition, sensor information relating to the sensor, and process workflow information; a user interface comprising a display depicting a bioproduction workspace, wherein the bioproduction workspace comprises a process workflow title and a plurality of bioprocess modules; and a central processing unit in electronic communication with the sensor, memory and user interface.