This nitrogen recovery method is for causing nitrifying bacteria to decompose an ammonia component in an ammonia-containing gas, and recovering a nitrogen component contained in ammonia as an ammonia gas decomposition product, involving: supplying circulating water to a microorganism decomposition tank retaining a nitrifying bacterium carrier carrying nitrifying bacteria to maintain the carrier wet; passing ammonia-containing gas through the carrier in the wet state in an oxygen-containing atmosphere; dissolving an ammonia component in the ammonia-containing gas in the circulating water, together with an ammonia gas decomposition product produced by the nitrifying bacteria, to continue decomposing the ammonia-containing gas while the decomposition product is accumulated in the circulating water; and collecting all or a portion of the circulating water to recover the ammonia gas decomposition product, when the concentration of nitrate ion as an ammonia decomposition product in the circulating water reaches a predetermined concentration of 5000 mg/L or more.

Electrochemical sensing devices and methods of using thereof employs a set of one or more sensor-integrated sampling wells or containers that operates with a pressure differential micro-valve to move controlled volume of sampled fluid within a controlled cell-growing environment. The differential micro-valve can be integrated into an instrumented well having one or more sensors to provide a high-throughput smart well plate platform for use in automation operation in diagnostics and drug discovery.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present application relates to a bioreactor system for culturing cells, especially cells without cell walls; the bioreactor system includes: a container containing a hollow cylinder with a diameter of D1 and a height of H1, and a hollow circular truncated cone with an upper diameter of D2, a lower diameter of D3, and a height of H2, where the hollow cylinder is connected to a top surface of the hollow circular truncated cone, and D1=D2; an oscillator, configured to cause the container to make an eccentric motion according to a certain eccentricity and rotational speed; a ventilation device, configured to introduce an oxygen-containing gas from an upper portion of the container to the inside of the container, and a culture solution filled in the container, of which a top surface is exposed to the oxygen-containing gas; where the oscillator is configured to maintain the eccentric motion of the container, such that a ratio of the total liquid surface area to the volume (S/V) of the culture solution when in a steady state of motion is 5.65 or more, the turbulence kinetic energy is 2.73E−03 m.sup.2/s.sup.2 or more, and the flow field shear rate is 20.27/s or less, where the total liquid surface area is the sum of the contact area between the culture solution and the reactor wall surface and the contact area between the top surface and the gas.

An integrated two-phase anaerobic dry fermentation reactor based on a biomimetic principle of rumen includes a reactor body; wherein the reactor body includes a dry fermentation chamber, a secondary fermentation chamber, and a liquid storage chamber. The dry fermentation chamber is arranged at an upper portion of the reactor body. The liquid storage chamber is arranged at a bottom of the reactor body. The secondary fermentation chamber is arranged between the dry fermentation chamber and the liquid storage chamber in the reactor body. The dry fermentation chamber is connected to the secondary fermentation chamber by a porous structure.

An anaerobic digestion device based on self-sustained air flotation includes an anaerobic digestion tank unit, a self-sustained air flotation screening unit and a biogas measurement and collection unit. The self-sustained air flotation screening unit includes an air flotation screening part, a material sedimentation part, a reflux part and a three-phase separation part connected sequentially from bottom to top. A digested material in the anaerobic digestion tank unit is pumped into the air flotation screening part, overflows into the material sedimentation part, and then is raised to the reflux part. Gas passing through the three-phase separation part and gas produced in the anaerobic digestion tank unit enter the biogas measurement and collection unit to be measured and collected.

In a culturing method, microalgae are cultured in a culturing solution while a gas containing carbon dioxide is supplied to the culturing solution. In an ion concentration acquisition step, an acquired value of a concentration of hydrogen carbonate ions in the culturing solution is obtained. In an ion concentration adjustment step, in the case that the acquired value does not reside within a set concentration range that is set beforehand, at least one of a temperature or a pH of the culturing solution is adjusted, whereby the concentration of hydrogen carbonate ions in the culturing solution is adjusted to reside within the set concentration range.

A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5.

A methane generating device includes: a culture tank in which methane generating bacteria are cultured with a culture liquid; a gas feed device that feeds carbon dioxide and hydrogen gas required by the methane generating bacteria to the culture tank; and a generated gas extraction device that extracts methane generated by the methane generating bacteria.