A portable and modular renewable energy microgeneration apparatus is disclosed that includes at least four modular units. The first modular unit includes a mixing tank and a chopper. The second modular unit includes a buffer tank, a liquor tank, and a pasteurization tank that pasteurizes waste that has been mixed with liquid from the liquor tank by the mixer, chopped into smaller sized components by the chopper, and pre-warmed by the buffer tank. The third modular unit includes a digestion tank that performs anaerobic digestion on pasteurized waste received from the pasteurization tank. And the fourth modular unit includes a gas storage tank that stores gas generated by the waste in at least one of the mixing tank, the chopper, the buffer tank, the liquor tank, the pasteurization tank, and the digestion tank. Each of the four modular units is both portable and modular.

The invention provides a skin culturing apparatus configured for biphasic culturing of mammalian skin and/or a mammalian skin substitute. The apparatus comprises a first chamber that is configured to provide a gaseous environment having a relative humidity below 90% and comprising less than 5% CO.sub.2, and a second chamber that is configured to provide tissue culture medium at a temperature of 33.0-37.5° C. and pH of 6.1-7.9. The invention also provides methods for culturing mammalian skin and/or skin substitutes, as well as methods for testing mammalian skin and/or mammalian skin substitutes cultured in the skin culturing apparatus.

The present invention provides improved bioprocessing systems and methods for cell culture using the improved bioreactors, e.g., batch-fed or perfusion bioreactor cell culture systems for production of monoclonal or bi-specific antibodies, which are modified to include one or more membrane gas transfer modules in place of a sparger- or microsparger-based aeration systems to better regulate the levels of critical gases in a bioreactor cell culture, e.g., the dissolved levels of O2 and CO2, even at high cell densities, without subjecting the cells to bubble-burst associated cell death.

The present invention relates to collapsible and/or foldable fermentation and/or culture vessels fabricated of an adjustable frame including sections of gas permeable material or in the alternative a non-rigid gas permeable polymeric container or bag fabricated of either a gas permeable or non-gas permeable material with the further benefit of more efficient inventory space and disposal space, wherein the gas permeable material or non-gas permeable material comprises an analyte or pH sensing material integrated or impregnated into at least one section of the material.

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

The present invention is in the field of a method of producing lactic acid in high yield in a sequencing batch reactor. Therein glucose may be used as feedstock for bacteria, which ferment the glucose into lactic acid. The reactor is operated under at least partly defined non-axenic conditions and in a cyclic mode.

The invention relates to a comparison unit (130) configured for determining if a first pH measuring device of a first tank (104; 106) is affected by a pH-measuring problem, the comparison unit being configured for: —receiving a first CO2 concentration and a first pH value, the first CO2 concentration being a CO2 concentration of a first gas volume above a medium in a first tank, the first CO2 concentration and the first pH value being measured at a first time when the medium in the first tank is in pH-CO2 equilibrium state with the first gas volume and before said equilibrium state is modified by the metabolism of a cell culture in the first tank, the first pH value being a measured value provided by a first pH measuring device operatively coupled to the first tank (102); —receiving a second CO2 concentration and a second pH value, the second CO2 concentration being a CO2 concentration of a second gas volume above a medium in a second tank, the second CO2 concentration and the second pH value being measured at a second time when the medium in the second tank is in pH-CO2 equilibrium state with the second gas volume and before said equilibrium state is modified by the metabolism of a cell culture, the second pH value being a measured value provided by a second pH measuring device; —comparing the first and second pH values and CO2 concentrations for determining if comparing (206), by the comparison unit, the first and second pH values and comparing the first and second CO2 concentrations for determining if the first pH measuring device is affected by the pH-measuring problem.

Systems and methods for producing cultured food products such as cultured meat in a form of meat cut or offal are provided, comprising growing non-human-animal adherent cells on edible scaffold(s) in a cultivation system. The cultivation system typically comprises a plurality of cell culture bioreactors receiving medium at a controlled flow rate adjusted to nourish the non-human-animal adherent cells.

An information processing apparatus estimates a quality of an antibody produced from cells and a quality of the cells on the basis of a culture state of the cells, searches for the culture state of the cells that improves the estimated quality of the antibody and the estimated quality of the cells, and derives process conditions for cell culture in which a culture state of the cells is the searched culture state.