The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

Provided is a cell-culturing apparatus including: an incubator that can maintain an interior thereof in an environment that is appropriate for growth of cells; a cell-culturing vessel that is accommodated inside the incubator; an optical-data acquisition unit that acquires optical data of a medium in the cell-culturing vessel; a medium changing unit that changes the medium in the cell-culturing vessel; and a controller, wherein a timing at which the medium is changed is determined on the basis of a change over time in the optical data acquired by the optical-data acquisition unit.

The invention provides a lighting system (1000) for indoor microbiome management in an animal residence (200), wherein the lighting system (1000) comprises a light generating device (100), a control system (300), and an input system (305), wherein: the light generating device (100) is configured to generate first device radiation (111), wherein a spectral power distribution of the first device radiation (111) is selected for promoting persistence of the first microbes (7) relative to second microbes, other than the first microbes (7); the input system (305) is configured to receive and/or sense a microbiome influencing parameter and to provide a related input signal to the control system (300): the control system (300) is configured to control the light generating device (100) in dependence of the related input signal.

Methods and systems related to delivery of complex feed nutrients based on a number of cells are presented herein. A method of controlling a nutrient feed, a method of developing a feeding schedule, and a nutrient feed control system are presented herein. Volume of feed per day can be proportional to a predicted change in integrated viable cells (IVS) from the present feeding day to the next feeding day. A per cell factor (PCF) can be determined by determining a normalized feed per cell value for a time interval of a preliminary fed-batch bioreactor run in which the feed consumed is approximately equal to the feed provided. The volume of feed per day can be set equal to a product of the PCF and change in IVS from the present feeding day to the next feeding day.

A system for rapid multimodal detection of anaerobic SRB in a fluid test sample via integrative spectral comparison includes a compact, mini, or advanced assay reader with sample compartments configured to receive assay samples for comparison with respective spectral reference standards. The assay reader includes one or more excitation sources configured to emit light at wavelengths selected to facilitate colorigenic and/or fluorogenic analysis of the assay samples. The system includes a kit with one or more first reagents formulated to form a first assay sample indicating a level of H.sub.2S in the fluid test sample, and one or more second reagents formulated to form a second assay sample indicating a level of enzymatic activity of anaerobic SRB species. The kit also includes one or more oxygen scavengers to maintain an anaerobic environment.

It is an object to provide an observation method for improving culturing efficiency of cells and obtaining desired cells without cost.

The present technology provides a cell treatment system including a cell culture unit capable of culturing a cell group, a measurement unit capable of measuring the cell group, and an estimation unit, in which the estimation unit estimates, on the basis of information regarding a cell before treatment of the cell group and at least one of information regarding a predetermined cell to reach after treatment of the cell group or a predetermined treatment condition for the cell group before treatment, a treatment condition under which the cell group is derivable to the information regarding the predetermined cell or information regarding the cell after treatment of the cell group derived by the predetermined treatment condition.

Method or system for monitoring a condition of an organ, which may be a donor organ, using an optical device; providing a reservoir of normothermic fluid; providing a perfusion circuit for the normothermic fluid; and providing a pump in fluid communication with perfusion circuit. Providing an oxygenator for normothermic fluid during circulation through the perfusion circuit to perfuse the donor heart with oxygenated blood; an optical device integrated within the reservoir, or pump or oxygenator, or in-line flow-based within perfusion circuit through normothermic fluid flows. May use total internal reflection for optical device. Monitoring condition of an organ, circulation system for blood, exterior to a body, but connected to the body; and adding filtration to blood, and filtered blood goes back into body. Monitoring a condition of a heart or lung, using extracorporeal membrane oxygenation (ECMO) to monitor the oxygenation levels in circulation.

The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. ##STR00001## Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.

Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs.

Functionalized substrates with properties that can control cell-substrate interactions, induce cellular processes and decisions by means of passive and active control to enhance cell proliferation, cell migration and wound healing are provided. By including a sensing electrode on the substrate, it is possible to measure the metabolites of cells or follow the migration of additives like drug molecules in real-time. The present technology provides a new cell culture and imaging platform composed of a transparent and chemically and topographically customized latex film with electrodes that enable real-time measurement of e.g. pH and ion concentration in the cell medium as well as the metabolic states of the cells.