A cassette assembly (1) comprises a cover (100), two cassette halves (200A, 200B) and a slider (300) comprising multiple test chambers (302). The cassette halves (200A, 200B) comprise waste tanks (230) in fluid connection with reservoirs (240), prefilled in one of the cassette halves (200A, 200B) with test agents. The particular design of the cassette halves (200A, 200B) enable forming predefined 5 volumes of liquid to achieve predefined concentrations of the test agents in the reservoirs (240) in one of the cassette halves (200A, 200B) and liquid in the reservoirs (240) in the other of the cassette halves (200A, 200B). Gradients of the test agents can then be established over the multiple test chambers (302).

An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber.

A container for storing, mixing and/or cultivating a medium, in particular a bioreactor for a medium, comprises a line system as a discharge line, feed line and/or bypass line of the bioreactor. The system can comprise a line body formed as a single piece for a medium to flow therethrough. The has a first and a second connection region for connecting in particular to the container and/or the line system and at least a first and a second coupling apparatus in the region between the connection regions.

Provided herein are injection molded perfusion-enabled bioreactors and systems including said bioreactors. The bioreactor can include a lid, a frame comprising an an-ay of sample wells, a membrane adhered to a bottom of the frame beneath the array of sample wells, and a skirt adhered to a bottom of the frame, such that the membrane is located between the frame and the skirt. The skirt can include a plurality of channels corresponding to the sample wells. When the sample wells are filled with a 3D cell culture support matrix, a pressure above the 3D cell culture support matrix is atmospheric pressure and a pressure below the membrane is less than atmospheric, thereby perfusing a fluid along a vertical fluid flow path from the sample wells through the

The present disclosure relates to a bioreactor assembly having a housing defining an interior chamber; a lid assembly removably coupled to the housing and enclosing the interior chamber; and a gimbal assembly disposable within the interior chamber. The gimbal assembly includes a cradle configured to hold an organ or organ scaffold and an arm assembly configured to move the cradle between a plurality of positions. The bioreactor assembly also includes an ultrasound imaging unit positioned to capture volumetric and/or spatial data of the organ or organ scaffold.

Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation device having one or more cell scrapers.

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

Provided is a process and material including the operation of a reactor that applies kinetic energy, heat, and pressure are performed nearly concurrently to biomass feeding of biomass to create functional soil amendment with superior SOM creation and carbon capture.

The present disclosure provides a biomimetic cell culture apparatus that mimics interactions among organs in a human body. The present disclosure includes a plurality of culture units for culturing cells, a conduit for connecting the plurality of culture units to each other to form a circulating path, a pump unit disposed on the conduit for forming a flow in culture medium such that the culture medium circulates through the plurality of culture units, and an agitating module for agitating the plurality of culture units.

The present invention concerns the field of high-efficiency, quality-controlled production of blue-green algae for direct human consumption, for extraction of proteins, vitamins, and amino acids, and for production of organic materials loaded with the special isotope 13C.

It is an object of the present invention to describe a high-efficiency photobioreactor.