A pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides. In another embodiment, floating pistons mix the fluid, pushing it through a mixing plate with one or more apertures. In a third embodiment, the mixing device is a rotating drum with bubble-catching blades and rotating mixing plates with apertures. The top of the vessel for these mixers may include a closed top and sterile filters.

The present invention relates to bioreactor system and method thereof wherein support matrix (2) comprises at last one central shaft and plurality of peripheral shaft being radially surrounds central shaft. Arrays of discs (11) are mounted along the shaft by defining interspatial vicinities between two successive plates. Thus, discs mounted on peripheral shafts are rotated within the interspatial vicinity of discs of central shaft to ensures sufficient mixing and avoid stagnant fluidic zones which is created when discs are mounted closely apart from each other on shafts. Further, plurality of deflector vanes that are axially provided along the length of the central shaft to redirect substantially co-axial direction fluid flow into interior of culture vessel and more specifically towards the central axis. Thus, bioreactor system provides scalable and disposable bioreactor with efficient mixing and homogeneous conditions and thereby supports high density growth and maintenance of cells and other biological material.

This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.

According to the invention, there is provided a parallel bioreactor system, comprising: an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part, a cross section of the cylindrical portion is consistent with the cross section of the top of the inverted truncated conical bottom, and the bottom of the cylindrical portion is joined with the top of the inverted truncated conical bottom; disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating culture solution, wherein each disposable culture bag is provided with a multifunctional cover plate, and the multifunctional cover plate is connected to the top of the culture bag to seal the culture bag, and is provided with a plurality of connection holes leading to interior of the disposable culture bag; and a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in the disposable culture bags.

The cell culturing device includes a vessel that accommodates a culture solution containing a medium and cells, an agitator that is provided in the vessel and agitates the culture solution, a discharge pipe that discharges the culture solution in the vessel to outside the vessel, and an oxygen intake pipe that draws oxygen into the vessel. The discharge pipe has an end below the agitator in the vertical direction.

Method for operating an agitating device and a digester wherein the digester (1) is filled with a substrate (7) and wherein an agitating device (10) controlled by a control device (50) is disposed in the digester (1). The following process steps are carried out. a) A target load curve (60) is lodged in the control device (50) b) the control device (50) prescribes a target speed of rotation (61) c) the control device (50) operates the agitating device (10) at an actual speed of rotation (71) corresponding to the prescribed target speed of rotation (61); d) the control device captures an actual measurement value (81) which is characteristic of the torque of the agitating device (10) at the actual speed of rotation (71); e) the control device (50) derives from the actual measurement value (81) an actual characteristic value (91) of the applied torque of the agitating device (10); f) the control device (50) compares the derived actual characteristic value (73) against the target characteristic value (63) of the substrate (7) resulting from the target load curve (60) at the prescribed target speed of rotation (61); g) the control device (50) controls the agitating device (10) in dependence on the result of comparison.

A multi-mode magnetic treatment device combining a permanent magnetic field and an electromagnetic field includes a base, a sample stand, an electromagnetic mechanism, a permanent magnetic mechanism, a permanent magnetic mechanism motion drive unit, a sample stand ascent-descent drive unit and a control system. The sample stand, the electromagnetic mechanism and the permanent magnetic mechanism are mounted on the base. The electromagnetic mechanism and the permanent magnetic mechanism are connected to the control system. The permanent magnetic mechanism is mounted on the base through the permanent magnetic mechanism motion drive unit. The sample stand is mounted on the base through the sample stand ascent-descent drive unit. A magnetic field of the electromagnetic mechanism and a magnetic field of the permanent magnetic mechanism respectively act on the sample stand to apply a rotational permanent magnetic force and an alternating electromagnetic force to a sample.

A stirring apparatus including a stirring mechanism configured to execute a stirring process on cells in a culture vessel, and a control unit programmed to at least one of control the stirring mechanism, determine the stirring process of the stirring mechanism, and determine whether the stirring process is performed or not.

In an aspect, there is provided a computer-implemented method for approximating product production in a bioreactor. The method comprises: providing a bioreactor containing live cells in a substrate, the bioreactor for cultivating, over a time period, a product derived from or of the cells during a manufacturing process; providing two or more sensors for measuring respective two or more different physical attributes of the substrate during the time period; receiving, at a processor, sensor data from the two or more sensors; and determining via the processor, based on a predetermined or recursive algorithm that correlates the sensor data and the product production by independent consideration of the sensor data of the two or more different physical attributes or by multivariate consideration of the sensor data of the two or more different physical attributes, an approximate amount of the product.

The present invention provides a self-draining well plate cassette and an automated system for cultivating C. elegans comprising a housing, a well plate assembly having at least two of the self-draining well plate cassettes, a liquid dispensing assembly operated by a first three axes positioner, a wash and imaging assembly operated by a second three axis positioner, a reagent assembly, a pipette tip holder, and a controller. The present invention further provides a method of using the above-described system to cultivate C. elegans.