A bioreactor includes a base and a lid. The base includes two opposing curved or convoluted surfaces, two opposing flat surfaces, a window disposed on one of the flat surfaces, the window having a higher degree of transparency compared to other portions of the base, wherein images or videos of cells are captured through the window by non-invasive ISM device, and a rounded bottom. The lid is attachable to the base. The lid includes a shaft and a semipermeable membrane attached to the shaft, the semipermeable membrane being permeable to oxygen but impermeable to viruses and bacteria. The PAT-based online analysis directs the adaptive manipulations of bioreactor towards efficient, automated, and GMP-compliant clinical protocols of cell culture.

An acoustic resonator device includes a fluid chamber with a carrier surface and a reflector surface. The fluid chamber is filled with a volume of a fluid that includes a phase-separate material such as algae. The carrier surface is coupled to a transducer, which may be a lead zirconate titanate (PZT) transducer. The transducer, when supplied with electricity, emits an acoustic wave-based output of an output frequency (e.g., ultrasound). A sensor may be used to track one or more eigenfrequencies of the volume of fluid, the acoustic resonator device, or some combination thereof. A controller may receive tracking data from the sensor and control the voltage source, the transducer, or some combination thereof to ensure that the output frequency matches one of the tracked eigenfrequencies, thereby maximizing excitation of the fluid to improve efficiency of mixing, stimulation, and separation of materials from fluid (e.g., for algal biocrude production).

The present invention relates to a bioreactor assembly comprising a plurality of trays (30) for holding a cell culture bag, a holder (2) on which the trays are mounted side by side to form a first series of trays rocking mechanism (4) for rocking the trays, the rocking mechanism (4) being operatively connected to each of the trays (30).

The invention also relates to a bioreactor system (100) comprising at least one bioreactor assembly.

A method for tuning optical measurements on continuously mixed reactors, wherein a content of the reactor has at least one optically detectable measured variable, which is carried out by at least one optical measuring arrangement, wherein at least one optical measurement is tuned to a form, distribution, or movement state of at least one phase of the reactor content, wherein mixing the reactor content causes local changes in the permittivity within the reactor, which is detected at at least one location having a known distance from the at least one optical measuring arrangement to be tuned based on a permittivity signal, and wherein the detected permittivity signal of at least one location having a known distance from the at least one optical measuring arrangement to be tuned is used to tune at least one optical measurement to the form, distribution, or movement state of the reactor content.

A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

Provided is a cell detachment device for detaching a cell on a substrate from the substrate by applying vibration, the cell detachment device including: a vibration member configured to be vibrated by a drive voltage at an amplitude depending on an output value of the drive voltage; a vibration transmission member configured to vibrate by receiving vibration of the vibration member, and transmit the vibration of the vibration member to the substrate; a drive voltage output unit configured to output the drive voltage; an amplitude detection unit configured to detect an amplitude of vibration of the vibration transmission member; and a drive voltage control unit configured to control the output value of the drive voltage, based on the amplitude detected by the amplitude detection unit. A cell detachment method which uses the cell detachment device is also provided.

An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

A bioreactor consumable unit (50; 500) comprises a bioreactor part (60); a fluid feed container part (80) integrally connected with the bioreactor part and including at least one fluid feed container (82) in fluid communication with the bioreactor (60); and an integral pumping element (100, 110; 160, 206) configured to enable fluid to flow from the at least one fluid feed container (82) to the bioreactor (60). The bioreactor part (60) includes a bioreactor chamber (62) and a stirrer (64) for agitation of a cell culture (66) in the chamber. The pumping element comprises a combination of a syringe pump (110) and an associated three-way valve (102). The bioreactor consumable unit (50; 500) may be inserted into a receiving station (20) of a cell culture module (10) for the processing and control of a bioreaction in the bioreactor chamber (62). The provision of the fluid feed containers (82) and the pumping element (100, 110; 60, 206) as integral parts of the bioreactor consumable unit (50; 500) facilitates the set-up of the processing, because the various fluid connections between those components are already established. The syringe pump (110) provides accurate dispensing of fluids to the bioreactor chamber (62).

A chemostat comprises a chamber containing media with cells; an input tube for delivering nutrient-laden media that support cell growth and division within the chamber at an inflow rate; and an output tube for withdrawing a sample from the chamber at an outflow rate. The inflow and outflow rates are regulated such that the chemostat is operable in a sample accumulation phase in which the outflow rate is zero and the inflow rate increases in proportion to an instantaneous volume of media in the chamber so as to accumulate the sample without changes in a metabolic state within the chamber, or a sample withdrawal phase in which the outflow rate is regulated at a higher rate than the inflow rate to withdraw the accumulated sample from the chamber rapidly at one time and the inflow rate remains in proportion to the instantaneous volume to maintain chemostasis in the chamber.