A cell culture device (200) includes a cell culture vessel (22) for culturing cells, a factor container (81) for storing a factor, a reagent container (82) for storing a reagent for introducing the factor into the cells, and a flow path for transporting the factor and the reagent from the factor container (81) and the reagent container (82) to the cell culture vessel (22).

A movable cell incubator contains: a body, a first lid, a second lid and an electric control unit. The body includes a first internal space, a refrigeration room, and an airtight culture room. The first lid airtightly covers the culture room, the second lid airtightly covers the refrigeration room, and the control unit includes a microprocessor, a power module, a digital/analog conversion module defined between a microprocessor and the power module, a heating module controlling temperature of the culture room, a cooling module supplying cold source to the refrigeration room, a peristaltic pump module, a flow sensing module, a CO2 detective supply module supplying CO2 to the culture room, and a setting display module exposing and fixed on the first lid, with the peristaltic pump module aseptically connected between cell culture media and cell culture bag by multiple conveying tubes.

The present invention relates to methods and systems for performing perfusion cell culture whereby the supernatant of the bleed stream is recovered.

Disclosed herein is an instrument suitable for processing cells for example culturing, concentrating or washing said cells, the instrument comprising: a housing for accommodating mechanical elements including at least one fluid pump; and a disposable processing kit complementary to the mechanical elements within the housing and comprising a fluid circuit including a fluid reservoir and plural fluid paths capable of carrying fluid flow caused by said pump(s), the instrument further including a mechanism for determining the quantity, or change in quantity of the fluid in the reservoir resulting from said fluid flow, the instrument yet further comprising a controller operable to control at least the pump and operable to perform a fault determination process, which includes the steps of determining the expected flow rate of said pump(s) calculated from the speed of the pump(s) and comparing that expected flow with the change in quantity of the fluid in the reservoir as determined by said mechanism.

Provided is an apparatus for inducing and/or controlling flow of a fluid within a microchannel in a microfluidic device. The apparatus includes a fluid reservoir configured for holding a volume of fluid to be transported through said microfluidic channel and also configured for fluid connection to an inlet of said microfluidic channel. The apparatus also includes an evaporation reservoir configured for fluid connection to an outlet of said microfluidic channel. The evaporation reservoir includes at least one wetting, wicking or hydrophilic structure positioned at least partly within the reservoir. The wetting, wicking, or hydrophilic structure is capable of absorbing or conducting a fluid present in the microfluidic channel via wicking action or capillary force and maintaining a substantially constant volume of fluid in the evaporation reservoir. In use, evaporation of fluid at the outlet results in fluid being drawn from the fluid reservoir through the microfluidic channel to thereby create a flow of the fluid in the microfluidic channel.

Aspects of the present disclosure relate to systems and methods for manufacturing biologically-produced pharmaceutical products. Some of the systems described herein comprise an upstream component comprising a bioreactor and at least one filter (e.g., a filter probe) integrated with a downstream component comprising a purification module comprising at least a first partitioning unit and a second partitioning unit. In some embodiments; these integrated biomanufacturing systems may be operated under continuous or conditions and may be capable of efficiently producing pure, high-quality pharmaceutical products.

The purpose of the present invention is to efficiently dispense cells while reducing the risk of incorporating bacteria, etc. A cell dispensing device 1 includes: a first tube 23a which connects a container 21 and a dispensing container 22; a syringe pump 24 attached to a second tube 23b connected to a branching portion 31 formed on the first tube 23a; and a buffer tank 25 provided between the syringe pump 24 and the branching portion 31. In addition, a cell suspension contained in the container 21 is temporarily stored in the buffer tank 25 by means of the syringe pump 24, and the cell suspension stored in the buffer tank 25 is delivered to the dispensing container 22.

This disclosure describes hardware for microfluidic chips and an associated support module for facilitating operation of one or more microfluidic chips. The microfluidic chips described herein are designed for supporting multiple different tissue types, including kidney tissue, liver tissue, adipose cells, and so forth. Chip geometry facilities fluid flow through one or more channels of the chip with a particular flow rate. For example, shear forces are reduced where needed to ensure proper flow rate of fluid in the channels. The chamber geometry and the geometry of the channels ensures that a desired amount of oxygen is delivered to sample cells or tissues in a controlled manner.

A Disposable Bioprocess System having a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating the sterile Disposable Bioprocess System in perfusion mode for continuously processing.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.