The present invention relates to methods and apparatus for microbial conversion of carbon dioxide, carbon monoxide and hydrogen to methane, and to the microbial populations, and the media comprising the microbial populations, that may be used in such methods and apparatus.

Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.

A tissue processing apparatus, a filter and a method for processing tissue therefrom #! The problem to be solved is to provide a portable apparatus for processing harvested fat to form a good quality graft that does not clog syringes, and the problem is solved by providing an apparatus as in the present invention with a filter that is inclined to the base of the apparatus and comprises of plurality of elongated protrusion that are structured at an angle on the surface of the filter and blocks the fibres when the harvested fat moves on the inclined surface of the filter, thereby filtering the harvested fat and avoiding clogging of syringes.

A dynamic incubator system and method for mammalian cells. The dynamic incubator system includes an incubator and a programming device communicatively coupled to the incubator. The programming device includes a memory, one or more processors, a display, and an input mechanism. The programming device is adapted to enable at least one preset temperature and gas concentration sequence to be one or more of designed for the interior of the incubator, saved to the memory of the programming device and/or selected for implementation within the interior of the incubator. The at least one temperature and gas concentration sequence includes programmed changes to the temperature and a CO.sub.2 gas concentration. A purging mechanism is coupled to the incubator and releases a portion of a gas concentration to enable rapid changes in the temperature and gas concentration in the incubator.

An object of the present invention is to provide: an algae water concentration system that efficiently concentrates algae water in a culture pond into algae water containing algae having a desired size, with a simple structure and at low cost; and a method for operating the same. A cultured algae water concentration system 100 comprises: an algae water supply unit 17 that has an algae water supply container 18 that stores algae water sent from the culture pond therein, and a supply container outlet port 19 through which the algae water is taken out from the algae water supply container; and an algae water concentration unit that has a concentration container 1 for receiving and concentrating the algae water from the algae water supply unit, a filter 3 which divides the concentration container into upper and lower spaces and does not pass algae having a predetermined size or larger therethrough, a vibration device 5 that vibrates the filter in an out-of-plane direction, a concentration container algae water inlet port 7 which takes in the algae water to the concentration container and is arranged below the filter of the concentration container, a concentrated algae water outlet port 9 that is arranged below the filter of the concentration container and takes out algae water therethrough that has been concentrated in the concentration container, and a filtered water discharge port 8 that is arranged above the filter of the concentration container and discharges filtered water having passed through the filter.

Bioreactor systems and controlled operation of bioreactor systems are disclosed herein. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems. The controlled operation of the parameters includes, for example, cell concentration; a rate of flow; a volume; a pH; a temperature; a level of oxygen; a level of carbon dioxide; a level of bicarbonate ion; nutrient compound; and any combination thereof.

One embodiment provides a device for processing at least one biological sample capable of containing at least one target microorganism within at least one container. The device having at least one displacement device for generating the displacement of the contents of the at least one container and at least one site for receiving the at least one container. Additionally, the at least one container can receive the at least one biological sample within the at least one container, the container being delimited by a wall fixed on a base. Further, the at least one displacement device may be movable with respect to the base, and the at least one container may include a flexible material which allows the at least one container to be compressed against said wall.

Described herein is a surge tank based system to control surge tanks in a manufacturing train. The system includes data acquisition unit, data normalization unit, deviation identification and handling unit and an real time execution of control action from centralized computer in a manufacturing process unit, wherein said manufacturing unit includes an integrated system which incorporates a plurality of pumps, surge tanks, and weighing balances, and other unit operations alongside the normal unit operations of production of output product.

Provided are an apparatus and method for reducing the phenol concentration in a commercial enzyme solution and/or feedstock.

Devices and methods for isolating and characterizing microbial cells from an environment are provided. The devices integrate sub-micron constrictions in a nanofluidic device with a standard microtiter plate format to facilitate the high throughput isolation, culturing, analysis, and screening of bacteria and other microbial cells in natural and man-made environments, particularly environments containing microbes adhered on particulate matter.