The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

The invention relates to a bioprocess container (10) having an optical measuring device (100) for non-invasive spectroscopic measurement comprising: a container housing (12), a port housing (102), which is connected to the container housing (12) and is sealed off with respect to the interior (18) of the container housing (12); at least one radiation-emitting element (124), which is designed to transmit electromagnetic radiation through the at least one fluid contained in the container housing (12); at least one radiation-receiving element (126), which is designed to at least partly receive the radiation which was transmitted by the radiation-emitting element (124); and at least one measuring insert (122), which holds and supports the at least one radiation-emitting element (124) and/or the at least one radiation-receiving element (126).

The present invention relates to a pillar structure for a biochip. The pillar structure for a biochip according to the present invention is provided to form a biochip for analyzing a sample, which is a biological micromaterial such as a cell, together with a well plate configured to receive a culture solution, and the pillar structure includes: a pillar part having a seating surface on which a cell for culture is placed, and a light irradiation surface configured to be irradiated with light for observing the cell placed on the seating surface; and a plurality of holder parts extending in a state of being spaced apart from the pillar part and having an opening between the adjacent holder parts so that the culture solution is introduced between the holder parts.

A method for manufacturing a skin chip according to an exemplary embodiment of the present disclosure may include: a step of forming first and second PDMS layers disposed on both surfaces of a porous membrane and each having a microfluidic channel through which a culture medium is transferred to both surfaces of the porous membrane; a step of forming first and second MEA substrate layers disposed on the outer surfaces of the first and second PDMS layers, respectively, and having metal electrodes for measurement of TEER arranged at positions corresponding to the channels; and a step of forming first and second PMMA layers disposed on the outer surfaces of the first and second MEA substrate layers, respectively. In the method for manufacturing a skin chip according to in an exemplary embodiment of the present disclosure, the porous membrane may be made of a polycarbonate having pores of a predetermined size.

The present invention provides a cell counting and culture interpretation method and its application, which includes: obtaining a cell culture image; segmenting the cell culture image by a cell inference model to obtain a plurality of regions corresponding to a plurality of classification parameters; calculating a culture parameter corresponding to one of the classification parameters; and determining to replace a culture medium when the culture parameter is between 0.05 and 0.15 and determining to harvest cells when the culture parameter is greater than 0.69. The present invention can provide objective and consistent standards to further improve efficiency and reduce manpower costs.

A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

The invention relates to a device designed for the cultivation and radiation-induced killing of living biological cells. The device comprises a flat substrate and a functional layer for creating a wound in biological cells, said functional layer being applied to the flat substrate. The functional layer contains at least one photosensitizer which is designed to convert triplet oxygen into singlet oxygen by the application of electromagnetic radiation. As a result, biological cells on the functional layer can be killed by irradiation of low-intensity electromagnetic radiation. A wound can be introduced into a cell layer at a locally defined point easily, quickly, carefully, and in a flexible and cost-effective manner and thus the healing of the wound can be studied. The invention further relates to uses of the devices and a method for analyzing a migration and/or wound healing behavior of biological cells.

Systems and methods are provided for provided for automatic evaluation of sperm morphology. An image of a semen sample is obtained, and at least a portion of the image is provided to a convolutional neural network classifier. The convolutional neural network classifier evaluates the portion of the image to assign to the portion of the image a set of likelihoods that the portion of the image belongs to a plurality of output classes representing the morphology of sperm within the portion of the image. A metric is assigned to the semen sample based on the likelihoods assigned by the convolutional neural network.

A method for characterizing contractions in cell cultures of contractile cells, the method comprising: acquiring a series of images of the contractile cells; for each image in the series of images, computing a movement index characterizing the rate of change in mean absolute pixel intensity variations across the whole image; and using the movement index to assess the contractions of the contractile cells. Also, applications of the method to the study of cardiac tissue analogs and other cell cultures, and a culture system used to implement the method.

A method of determining a distribution of stein cells in a cell image, an electronic device and a storage medium are disclosed. The method acquires a cell image and segments the cell image and obtaining a plurality of sub-images. The plurality of sub-images is inputted into a stein cell detection model to detect to obtain a number of stein cells in each sub-image. A position of each sub-image in the cell image is determined. A distribution of the stein cells in the cell image is output, according to the number of stein cells in each sub-image and the position of each sub-image in the cell image. The present disclosure an accuracy of the distribution of stein cells in the cell image.