The present disclosure relates to methods and apparatus for measuring of multiple physiological properties of biological samples, such as measuring biological flux.

A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.

A method is provided which noninvasively monitors cells and which can accurately determine the progression of differentiation of the cells. This method, for evaluating the differentiation state of cells during culturing for inducing undifferentiated pluripotent stem cells to differentiate into desired cells, determines the progression of induced differentiation using any of the metabolites of glycolysis or any of the metabolites of the tricarboxylic acid cycle (TCA cycle), the metabolites being selected from among two or more types of amino acids contained in the culture solution or components in the culture solution derived from metabolism of the cells.

The present invention provides cell assay systems and its use in cell based assays.

A culture device is applicable to measurement of an electrical resistance of cells. The culture device includes a measurement chamber, a permeable layer, an upper working electrode, and a lower working electrode. The measurement chamber is longer in a first direction than in a second direction intersecting the first direction. The permeable layer partitions the measurement chamber into a first chamber on an upper side and a second chamber on a lower side. The permeable layer is permeable to liquid. The upper working electrode is disposed on the upper side with respect to the measurement chamber, and has working electrode portions longer in the first direction than in the second direction. The lower working electrode is disposed on the lower side with respect to the measurement chamber, and has working electrode portions longer in the first direction than in the second direction.

A microscope observation system comprises a cell production device provided with a culture vessel in which cells or cell masses are cultured, a microscope device capable of observing an object including cells or cell masses, a conveying apparatus that conveys the microscope device to the cell production device, and an operating control section that sends a command to the conveying apparatus to temporarily move the conveying apparatus from a current position to a relay position and then to move the conveying apparatus to a target position when focusing the microscope device onto the object, wherein a first distance from the current position to the relay position is a distance necessary to drive an actuator by at least a predetermined amount in order to actuate a rotation shaft or a translation shaft of the conveying apparatus, and a second distance from the current position to the target position is a shorter distance than the first distance.

The present invention is related to a biochip and production method thereof. The biochip comprises a carrier, a cell or tissue culture area deposited on the carrier, and a sensor area deposited on the carrier adjacent and fluidly communicating with the cell or tissue culture area. A containing space is contained in the cell or tissue culture area comprising a simulated vascular channel, a cell or a tissue and a culture medium. At least one sensor fixation area is contained at the sensor area for placing a sensor element. The present invention can be a model for stimulating cancer of specific patient to realtimely reflecting the cancer formation, transferring status and treatment strategies. The biochip could also carry testing drugs to observe how the drugs functioning to the cells/tissue as to provide a more accurate instruction of the drugs. The present invention can perform multiple test just within on chip which can save cost and also provide a more accurate test model for the patient.

A bioreactor system (1) has a pre-sterilized single-use bioreactor (2) with a reactor wall (20) surrounding an interior chamber (21) that receives a fluid medium (M). A sensor (3) for detecting an analyte in the medium has a sensor housing (30) with an outside thread (31) and a sensor shaft (32), a distal end portion (33) of which has an end face (34) with a region (35) permeable to the analyte. A sensor receptacle (4) connected to the reactor wall receives the sensor, to maintain the sterility of the single-use bioreactor. A circumferential flange (40) fixes the sensor receptacle to the reactor wall. The flange has an inside thread (42) that receives the outside thread. A wall (43) of the sensor receptacle is connected to the flange, and together with the sensor shaft, protrudes into the interior chamber, separating the sensor from the interior chamber.

The present disclosure relates to a semiconductor device and a cell potential measuring device capable of improving measurement accuracy of a potential of a solution.A semiconductor device includes a read electrode that reads a potential of a solution, a differential amplifier, a first capacitor connected in series in a loop feeding back an output of the differential amplifier to a second input different from a first input from the read electrode, a resistance element connected in parallel with the first capacitor, and a second capacitor connected between a reference electrode indicating a reference potential and the second input. The present disclosure can be applied to, for example, a cell potential measuring device.