The present invention relates to the use of online biomass capacitance monitoring in cultures as a way to control the growth of cells through the use of a temperature control loop. In certain embodiments, a biomass capacitance probe is used to measure the cell density, and a predetermined growth curve is used to adjust the temperature in the culture.

The present disclosure provides a system, including methods and apparatus, for culturing, monitoring, and/or analyzing organoids. In an exemplary method of organoid culture, the method may comprise disposing a scaffold in a receptacle having an open side. A sealing member may be bonded to the open side of the receptacle to create a chamber. An organoid may be formed in the chamber using the scaffold. Fluid and/or at least one substance may be introduced into the chamber from an overlying reservoir for contact with the organoid.

A millifluidic device for cultures of biological agents comprising: a main body (11) comprising at least a first hole (40) closed at the bottom; a separator (35); a membrane (36) fixed to said separator (35); a plug (15) closing said first hole (40); said separator (35) designed to be placed in said first hole (40); said separator (35) being extractable from said first hole (40); said membrane (36) divides said first hole (40 ) into an upper half-chamber and a lower half-chamber; a pair of tubes (25) to perfuse said lower half-chamber; a pair of tubes (26) to perfuse said upper half-chamber; a first slide (22) placed centrally on said plug (15); a second slide (42) placed centrally on said first hole (40); a cylindrical body (41) rises from said first hole (40) and said second slide (42) is placed on the top of said cylindrical body (41); said cylindrical body (41) has a second hole (43), coaxial to said cylindrical body (41).

An information processing apparatus that executes processing of obtaining, from an interference fringe image that is a two-dimensional distribution of intensity of interference fringes of object light and reference light, a phase difference image that is a two-dimensional distribution of a phase difference between the object light and the reference light, and obtaining a shape of an object to be observed based on the phase difference image includes at least one processor configured to acquire object-related information regarding the object to be observed, read out, from a storage unit in which the object-related information and a shape profile indicating the shape of the object to be observed are stored in association with each other, the shape profile corresponding to the acquired object-related information, and perform phase connection with respect to the phase difference image with reference to the read-out shape profile.

A cell area extraction unit (241) extracts a cell area in a phase image that is created based on a hologram obtained by in-line holographic microscope (IHM). A background value acquisition unit (242) obtains a background value from phase values at a plurality of positions outside the cell area. An intracellular phase value acquisition unit (243) averages a plurality of phase values on a sampling line set at a position close to the periphery of a cell, while avoiding a central portion in which the phase value may be lowered in the cell area, to obtain an intracellular phase value. A phase change amount calculation unit (244) obtains the difference between the intracellular phase value and the background value. A phase change amount determination unit (245) compares the value of the difference with thresholds in two levels to determine whether the cell is in an undifferentiated state or an undifferentiation deviant state. It is thereby possible to automatically make a correct determination while removing the influence of a theoretical measurement error by IHM.

Spectral data indicating an intensity of electromagnetic waves, which have been emitted to a cell suspension including a cell and a culture solution and have been subjected to an action of the cell suspension, for each wave number or wavelength is acquired. Preprocessing is performed on the spectral data. A soft sensor, which receives processed data obtained by the preprocessing as an input and outputs state data indicating a state of the cell or the culture solution, is constructed by machine learning using a plurality of combinations of the processed data and the state data as training data. The processed data for the spectral data acquired for a cell suspension including a cell which is being cultured is input to the soft sensor, and the state data output from the soft sensor is acquired.

Systems and methods are provided for evaluating a tissue that utilize a resistance to represent pressure of a fluid or gas passing through the tissue and a capacitance to represent compliance of the tissue.

Disclosed is a device for mechanically characterizing an element of interest, for example an oocyte. The mechanical characterization device includes: a support receiving a container suitable for containing a liquid medium; a holder for holding the element of interest; an indenting member; a magnet for generating a magnetic field in which the indenting member is intended to move and which participates in suspending the indenting member with an unstable horizontal direction oriented coaxially to the longitudinal axis; a controller to control the magnet to maneuver the indenting member in translation along the unstable horizontal direction; and a component for determining the mechanical characteristics of the element of interest.

Provided is a cell culture test device including a plurality of well units. Each of the well units includes an inlet portion through which a first fluid and a second fluid are introduced and a bottom portion including a first sub-well in which the first fluid is accommodated. A first stepped portion protrudes toward the center of the well unit along the circumference of the inner wall of the well unit such that the well unit has a lower portion whose inner width is smaller than that of an upper portion at the inlet portion side.

Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.