Provided is a cell analysis device including: a display unit; a storage unit; an analysis result input receiving unit to receive inputs of culture conditions and measured values of items obtained by analyzing a cell cultured under each condition, and store them in the storage unit associating the measured values with a corresponding one culture condition; a culture condition designation input receiving unit to receive input of designation of the culture condition; a correlation evaluation unit to read out measured values of items on the cell cultured under the designated culture condition from the storage unit, and evaluate a magnitude of correlation between the measured values of two items for all combinations of two of the items; and a correlation item presentation unit to display, on the display unit, a predetermined number of combinations extracted from among all the combinations based on an evaluation result by the correlation evaluation unit.

A three-dimensional (3D) microelectrode array device for in vitro electrophysiological applications includes a substrate and micro vias extending from the bottom face to the top face of the substrate. A microneedle at each micro via extends from the bottom face upward beyond the top face and forms a hypodermic microneedle array on the top face. Metallic traces on the bottom face interconnect the hypodermic microneedles to form the 3D microelectrode array. A microheater is positioned on the bottom face of the substrate. Microfluidic ports may be formed at the substrate. Interdigitated electrodes may be formed at the substrate.

A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

The present invention relates to a flatbed air-liquid interface exposure module for exposing a plurality of cells at an air-liquid interface to nanoparticles, the flatbed air-liquid interface exposure module comprising a moisturizing section, an exposure section, and an aerosol duct. Further provided are related systems and methods.

A method for measuring process parameters in liquid cultures in a plurality of microreactors of at least one microtiter plate includes continuously agitating the liquid cultures using an orbital agitator at least until the reaction is completed in all the microreactors. In order to allow process parameters also of such substances which themselves do not have any fluorescence activity to be measured with relatively low complexity and within a short time, 2D fluorescence spectra are recorded in a plurality of in particular different liquid cultures in the microreactors of agitated microplates. A device for carrying out the method is also disclosed.

Even for the case where cells such as human epidermal keratinocytes form a dense colony, or the case where cell contours are indefinite, each of the cells is automatically tracked with high precision, and behavior of each cell is analyzed with good precision. There is provided a method for analyzing behavior of a cell, which comprises a detection step of detecting positions of a plurality of cells for every frame of time-lapse images, while determining whether a candidate region extracted from the frame is a cell region by using a dictionary containing image data of cell nuclei; and a tracking step of tracking each cell by using a state space model using position of a most adjacent cell within a predetermined distance from a predicted position as observation data. When any cell is not found within a certain distance from the predicted position, data are considered missing.

The invention relates to a method for in vitro characterization of a eukaryote biological microtissue by imaging by means of a phase measurement technique without a reference beam, and the use of this method in vitro characterization in particular for: monitoring the quality of a microtissue, measuring the increase of the biomass of a microtissue during its growth and/or its amplification following the differentiation and/or the organization of a microtissue during its maturation, determining the phenotype of cells of the microtissue, and/or confirming the absence of non-differentiated cells in the microtissue.

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

A method of detection of a status of cells within a cell culture is described. The method is based on comparing an IR spectrum of a test sample obtained from the cell culture with an IR spectrum of a control sample or samples or with an IR spectrum of a sample or samples obtained at an earlier time point in the cell culture and correlating differences between the spectra with the status of the cells. The status may be classified into healthy or unhealthy. The test sample may or may not contain cells. The test sample may contain cell fragments, cell components or cell media, or may be cell supernatant. The comparison may be performed using a predictive model based on pattern recognition algorithms, such as support vector machines SVM, linear discriminant analysis LDA, principal component discriminant function analysis PC-DFA, neural networks, or random forests). The analysis results may be used to monitor and/or control the cell culturing process.

A system for evaluating stem cell differentiation includes a storage configured to store a machine learned model that has learned success or failure of cell differentiation for a combination of a stem cell and a differentiation induction method, an acquisition unit configured to acquire a target cell image that is an image of a target cell that is a stem cell to be induced to differentiate and differentiation induction information that is information related to a differentiation induction method applied to the target cell, and a processor configured to output differentiation success or failure information indicating an inference result related to success or failure of differentiation of the target cell into a desired cell type on the basis of the target cell image, the differentiation induction information, and the machine learned model. The differentiation induction information includes information indicating the type of a stimulus given to the target cell.