The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

The present invention relates to the use of online biomass capacitance monitoring in cultures as a way to control the growth of cells through the use of a temperature control loop. In certain embodiments, a biomass capacitance probe is used to measure the cell density, and a predetermined growth curve is used to adjust the temperature in the culture.

The invention comprises one or more gas volume fraction measurement devices operatively connected to one or more controllers and one or more deaeration mechanisms which receive control signals from said one or more controllers and perform an act on the system, such as by controlling a level of deaeration chemistry into some portion of the fermentation system. In one embodiment, the deaeration mechanism is an antifoam feed pump which pumps antifoam chemistry into a feed line of the fermenter in response to the measured gas volume fraction in the fermenter's recirculation loop, in an amount determined by the controller to be effective to reduce foaming and lower column height in the fermenter.

A cell culturing system includes an enzyme sensor that detects a concentration of a predetermined component of a culture medium used for culturing of cells, a sensor flow path in which the enzyme sensor is disposed, and a bioreactor that performs culturing of cells while causing the culture medium to flow through the sensor flow path, and further includes a deterioration index calculation unit that determines a deterioration index of the enzyme sensor, based on a detected concentration value of the enzyme sensor, and a detection period during which the detected concentration value is detected, and a lifespan determination unit that detects whether or not an integrated value of the deterioration index has reached a threshold value, and thereby determines the lifespan of the enzyme sensor.

Systems and methods for automated cell culturing are disclosed. In some embodiments, one or more cell culture vessels are fluidly connected with one or more multiport valves and one or more fluid pumps. The fluid pumps may pump various fluids into and out of the cell culture vessels as necessary to support cell growth, routed by the one or more multiport valves. In some embodiments, one or more components may be removable from other components so that some components may be prepared and sterilized independently prior to usage.

A data management system according to an embodiment includes a first storage control unit, a second storage control unit, and a third storage control unit. The first storage control unit controls storing, in a memory, one or more culture conditions indicating conditions for culturing a cell. The second storage control unit controls storing, in the memory, one or more pieces of measurement data for the cell cultured based on any one of the culture conditions. The third storage control unit controls storing, in the memory, one or more pieces of the measurement data designated among the pieces of the measurement data and one or more of the culture conditions designated among the culture conditions, in association with each other.

A system and method for the production of microbial consortiums and by-product material is provided. A physical containment system comprising phase spaces arranged in a discrete order to favor specific biological reactions is also provided. Phase profiles and phase data sets include the pre-determined physical and biological parameters for the phase space transitions. Movement of material from one phase to the next is hydraulically balanced enabling working fluid to continuously move in a fixed direction and rate of flow. Continuous monitoring of phase profiles and phase data sets provide feedback to the system enabling alteration of the conditions in the system to control reactions therein.

A cell manipulation panel includes a pixel array defining multiple pixels, an insulating layer forming multiple vias, and a cell gap provided with a fluid medium having cells therein. Each pixel has a TFT and corresponds to a corresponding via. The TFT includes a gate electrode, a first electrode, and a second electrode partially exposed to the fluid medium through the corresponding via. For each pixel, in an operational mode, when the gate electrode is provided with an OFF signal and the first electrode is not grounded, the TFT is turned off, allowing one of the cells in the fluid medium to be captured in the corresponding via by a dielectrophoresis (DEP) force. When the gate electrode is provided with an ON signal and the first electrode is grounded, the TFT is turned on, and the second electrode is grounded to release the captured cell to the fluid medium.

A portable incubator system integrated to a mobile phone providing a real-time tracking of samples and data flow is provided. The portable incubator system allowing cells to be cultured, reproduced and characterized in real-time without a need for a commercial incubator and a microscope-camera system installed within the portable incubator system.

A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.