According to the present invention, an initial-stage test apparatus-culturing condition (parameter set) is determined (22) on the basis of a manual culturing condition (26) for a specific cell. Multiple apparatus-culturing tests (20) are run in accordance with multiple test apparatus-culturing conditions, including the initial-stage test apparatus-culturing condition. A test apparatus-culturing condition at the time point when the result of an apparatus-culturing test satisfies requirement specifications is set (40) in a culturing apparatus as a setting apparatus-culturing condition, and the culturing apparatus is set up by using said setting apparatus-culturing condition.

A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.

An information processing apparatus that executes processing of obtaining, from an interference fringe image that is a two-dimensional distribution of intensity of interference fringes of object light and reference light, a phase difference image that is a two-dimensional distribution of a phase difference between the object light and the reference light, and obtaining a shape of an object to be observed based on the phase difference image includes at least one processor configured to acquire object-related information regarding the object to be observed, read out, from a storage unit in which the object-related information and a shape profile indicating the shape of the object to be observed are stored in association with each other, the shape profile corresponding to the acquired object-related information, and perform phase connection with respect to the phase difference image with reference to the read-out shape profile.

The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

Methods and systems for managing a petri-dish. Embodiments herein disclose a RFID tag affixed on the petri-dish, wherein the RFID tag has a thin formfactor, so as not to interfere in the use and operation of the petri-dish and a sufficiently large readability range. Embodiments herein disclose methods and systems for RFID based asset tracking of petri-dishes in a laboratory/pharmaceutical/manufacturing environment, wherein the movement of the petri-dishes are tracked automatically with minimal manual intervention.

The present invention relates to an automatic culture device using a single-use closed system flow path for culturing a cell or a tissue, and realizes reduction in manufacturing cost and high integration property of a device. A cell culture system including an automatic culture device and an information processing device. The automatic culture device includes a plurality of types of closed system flow paths possible to be installed and removed, and a plurality of culture devices. The information processing device includes: an input device configured to receive, as an input, at least one piece of data selected from a group consisting of data of an identifier of a patient, data related to a transplantation method, data related to a type of cell, data related to the required number of cells, and data related to a treatment plan; an arithmetic device configured to select, based on the input data, from options of a cell culture method, the culture devices, and the closed system flow paths, the cell culture method, the culture device, and the closed system flow path to be used; and an output device configured to output a number of the closed system flow path to be used and a number of the culture device to be used.

A computer-controlled system designed for multi organ decellularization uses continuous organ perfusion for fast, efficient and reproducible organ scaffold preparation under sterile conditions, allowing organ storage for ready organ regeneration. A single organ version is designed to be used for a single organ recellularization using cell, stem cell and autologous cell of organ receiver solutions for minimal or no immunogenic response, mimicking endogenous organ preparation for patients needing transplantation.

A microelectrode for electroporating an individual cell or embryo that includes a substrate with an electrically insulated surface, a first electrode adjacent to the electrically insulated surface of the substrate, a second electrode adjacent to the electrically insulated surface of the substrate and separated from the first electrode a predetermined distance so as to form a channel, and a liquid medium situated within the channel. The liquid medium is capable of fluidic transport of the cell or embryo through or into the channel and capable of supporting an electric field. The first and second electrodes include surfaces substantially orthogonal to the electrically insulated surface of the substrate with an edge length that is less than or equal to a diameter of the cell or embryo. The predetermined distance may be 50% to 200% of the diameter of the cell or embryo.

A bioprocessing installation comprising a reservoir tank, one or more fluid lines, one or more receiving containers and an electronic control unit. The fluid line fluidically connects the reservoir tank to a receiving container. The liquid biological medium can be transferred from the reservoir tank through the fluid line into the at least one receiving container. The bioprocessing installation comprises a sensor arrangement with at least one gas bubble sensor, which sensor arrangement is configured to detect one or more gas bubbles or foam or separated phases in the liquid biological medium by a gas bubble sensor and to generate one or more corresponding sensor signals, and the electronic control unit is configured to monitor the transfer of the liquid biological medium from the reservoir tank in the direction of the at least one receiving container based on the sensor signals generated by the sensor arrangement.

Techniques for predicting an amount of at least one biomaterial produced or consumed by a biological system in a bioreactor are provided. Process conditions and metabolite concentrations are measured for the biological system as a function of time. Metabolic rates for the biological system, including specific consumption rates of metabolites and specific production rates of metabolites are determined. The process conditions and the metabolic rates are provided to a hybrid system model configured to predict production of the biomaterial. The hybrid system model includes a kinetic growth model configured to estimate cell growth as a function of time and a metabolic condition model based on metabolite specific consumption or secretion rates and select process conditions, wherein the metabolic condition model is configured to classify the biological system into a metabolic state. An amount of the biomaterial based on the hybrid system model is predicted.