A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, the fat including a fluid component that also includes an oily component, a blood component and/or sterile solutions and of a solid component that includes cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of forming an emulsion of the fluid component in the sterile washing solution by active and/or passive stirring and discharging the emulsion from the outlet through a density gradient.

A system and method for harvesting autologous tissue, mincing it into fragments that are visible and measurable when filtered, and delivering a portion of the tissue back into the patient without the need to directly touch the tissue. During the same surgical procedure, the tissue can be mixed with a biocompatible agent before introduction into the repair site.

An integrated lipoaspirate processing system is disclosed for mechanically processing adipose tissue into a therapeutic material that, in some embodiments, may be directly injected into a subject. The system includes an emulsification device that is used to emulsify the lipoaspirate sample. The emulsified lipoaspirate is then filtered by a microfluidic filtration device. The filtrate of the microfluidic filtration device is then processed in a microfluidic dissociation device. The integrated platform or system helps standardize hydrodynamic processing of lipoaspirate by producing predictable and consistent shear forces and enabling automation in clinical settings. Progressive processing through multiple devices in series enables optimal recovery of regenerative cells while preventing clogging.

Anaerobic digestion apparatus comprises a first chamber for retaining organic matter before and/or during anaerobic digestion and a second chamber for retaining organic matter during anaerobic digestion. The anaerobic digestion apparatus is configured to refrigerate or heat the first chamber to suppress methanogenesis in the first chamber. The anaerobic digestion apparatus comprises a controller programmed to regulate the anaerobic digestion process and to thereby reduce system perturbations. The flow of organic matter to the second chamber where methanogenesis is regulated. There is disclosed an inoculum for anaerobic digestion comprising Acetobacterium woodii and Methanosaeta concilii.

Systems and methods for growing vegetation are provided. The disclosed systems (100) and methods (1100, 1200) use rotating growth mats (102) and a cutting device (112). The rotating growth mats and cutting device can be coupled to an anaerobic digester (402) to generated methane gas using vegetation grown on the growth mats. The systems and methods may further use C artificial light sources (108) and a nutrients delivery system (110) to assist growth.

The present invention provides a stem cell manufacturing system comprising: a sending channel (20) through which a solution containing cells flows; an apparatus (30) which is connected to the sending channel (20) and transfers a pluripotency inducer into the cells to produce cells harboring the inducer; and an apparatus (40) which cultures the cells harboring the inducer to produce cell clusters consisting of stem cells.

The present invention relates to methods of cell culture clarification. In particular the present invention discloses methods of cell culture clarification useful in the manufacturing process of biopharmaceutical molecules, such as antibodies.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

Disclosed are methods and devices for processing lipoaspirate that include mechanically-processing harvested lipoaspirate in a liposuction filter canister. In some embodiments, the devices are liposuction devices that include a lipoaspirate processing unit for mechanically-processing lipoaspirate. The mechanical processing reduces the average size of adipose tissue pieces in the lipoaspirate without substantially rupturing lipocytes therein.

The present invention is a kit to be used for obtaining Adipose tissue-based stem cells, which especially is suitable for obtainment of Adipose tissue-based stem cells, which comprises a reservoir (1) through which the entrance of the adipose tissue, that is taken from a predetermined area of the individual, into the kit; characterized by comprising a first housing (2), which is located on the said reservoir (1) and enables the transmission of the adipose tissue into the kit, a second housing (3), which is located on the said reservoir (1) and which enables the second injector (40) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), a third housing (4) which enables the third injector (50) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), an empty body (6) which is fixed inside the reservoir (1), a first inlet (7) which enables the transmission of the adipose tissue into the body (6) by pushing of the injector, a passage (8) which can rotate its own axis and which enables the circulation of the adipose tissue that is inside the body (6) between a desired inlet inside the body (6) and another inlet which is positioned in a 90 degree angle to this inlet by this rotation movement, at least one cover (10) which enables the said movement of the passage (8) and covers the top of the reservoir (1), a second inlet (12) which comprises a first splitting element (11) that is obtained by alignment of a multiple of cutting elements (10), and a third inlet (14) which comprises at least one second splitting element (13) which enables the adipose tissue split when it enters to and exits from the injector and is obtained by alignment of a multiple of cutting elements (10).