A method and apparatus associated with aseptic processing and production of cells in a non-sterile enclosure apparatus without chemical biocides is disclosed, by controlling the level of humidity throughout the enclosure to 25% relative humidity (RH) or less, and preferably at 20% or 15% or less RH. In addition, the temperature is controlled to 37 C., and continuous gas flow is maintained in the enclosure. Colony forming units from microbial contamination detected by environmental monitoring within the enclosure are significantly reduced.

An industrialized protein production system using carbon-containing industrial gas includes a bacteria preparation system, a raw gas purification system, a water purification system, a bacteria separation system and a protein preparation system, wherein the bacteria preparation system is respectively communicated with the raw gas purification system, the water purification system and the bacteria separation system, and the protein preparation system is communicated with the bacteria separation system. By purifying the raw gas and the raw water and removing impurities from the raw gas and competing bacteria in the raw water, excellent raw materials and environment are provided for bacterial reproduction, which enable the raw gas to have high-efficiency fermentation, thereby increasing the yield of proteins.

Disclosed herein are methods, systems, and compositions for the uniform pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel, sugars, or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes at least one high pressure, steam impermiable plug and a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system.

A method and a system for processing waste material to form a biogas and/or ethanol are disclosed herein. The method comprises subjecting waste material to separation according to specific gravity, to thereby obtain a fraction which is a separated lignocellulose; and processing the separated lignocellulose to obtain the biogas and/or ethanol. The system comprises at least one separator configured for separating materials in waste material according to specific gravity to obtain a first fraction comprising a low density material and a second fraction comprising a high-density material; and a bioreactor or bioreactor system configured for processing the separated lignocellulose to thereby obtain the biogas and/or ethanol. The separator contains an aqueous liquid selected such that a portion of the waste material sinks and another portion does not sink upon contact with the aqueous liquid.

The present invention provides a process for producing a fermentation product from a lignocellulosic feedstock. The process comprises soaking a lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock. The soaked feedstock is at least partially dewatered and the at least partially dewatered feedstock is subjected to pretreating in the presence of sulfur dioxide, sulfurous acid or a combination thereof to produce a pretreated feedstock composition. The pretreated feedstock composition is fed to an enzymatic hydrolysis in which the concentration of dissolved solids fed to the enzymatic hydrolysis is at least 50% (w/w) of the concentration of dissolved solids in the pretreated feedstock composition. The cellulose in the pretreated feedstock composition is hydrolyzed with cellulase enzymes in the presence of the dissolved solids to produce glucose. The glucose is fermented to produce the fermentation product.

A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185 C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process.

Disclosed herein are methods and systems for the concentration of cells from a cell suspension into unprocessed tissue, such as adipose tissue. Also disclosed herein are systems for optimizing hydration of tissue and cell enriched grafts.

An ethanol production facility is provided for processing a plurality of fermentable feedstock materials into an ethanol end product through fermentation and distillation. The facility includes a fermenting stage for converting the plurality of fermentable feedstocks into an alcohol mixture including ethanol and water, and a distillation stage. The distillation stage distills the low concentration alcohol mixture into a high concentration alcohol distillate. A kernel feedstock processing stage is receives and processes a kernel containing feedstock, and includes a receiving station for receiving the kernel containing feedstock. A storage station is provided for storing the kernel based feedstock, and a mill is provided for milling the kernel based feedstock into a flour based feedstock. A conveyor is provided for conveying the milled floor based feedstock to the fermenting stage. A flour feed stock processing stage is operable in parallel with the kernel feedstock processing stage for receiving and processing a flour predominant feedstock. The flour feedstock processing stage includes a storage station for storing the flour for predominant feedstock, and a pneumatic conveyor for conveying the flour to the fermenting stage.

An ethanol production facility is provided for processing a plurality of fermentable feedstock materials into an ethanol end product through fermentation and distillation. The facility includes a fermenting stage for converting the plurality of fermentable feedstocks into an alcohol mixture including ethanol and water, and a distillation stage. The distillation stage distills the low concentration alcohol mixture into a high concentration alcohol distillate. A kernel feedstock processing stage is receives and processes a kernel containing feedstock, and includes a receiving station for receiving the kernel containing feedstock. A storage station is provided for storing the kernel based feedstock, and a mill is provided for milling the kernel based feedstock into a flour based feedstock. A conveyor is provided for conveying the milled floor based feedstock to the fermenting stage. A flour feed stock processing stage is operable in parallel with the kernel feedstock processing stage for receiving and processing a flour predominant feedstock. The flour feedstock processing stage includes a storage station for storing the flour for predominant feedstock, and a pneumatic conveyor for conveying the flour to the fermenting stage.

An apparatus for treating a biomass feedstock at a high temperature, includes a cooling device 90 for cooling a biomass treated liquid at a high temperature; an enzymatic saccharification tank 103 for saccharifying a cooled treated liquid 101B with an enzyme; a solid-liquid separation apparatus 112 for removing water-slightly soluble substances contained in a saccharide solution 104 taken out from the enzymatic saccharification tank 103 and a foreign substance removing unit 113 provided with a microfiltration (MF) membrane 113a; a dilution tank 132, disposed downstream of the foreign substance removing unit 113, for diluting the saccharide solution from which the water-slightly soluble substances are removed by adding water thereto; a water separation unit 116, provided with a reverse osmosis (RO) membrane 116a, for removing water 114 from the diluted saccharide solution so as to obtain concentrated saccharide solution 115.