A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

A device that allows for either fat graft preparation or cell fraction harvest is disclosed. The device includes a first centrifuge tube configured to receive and process a biological substance, the first centrifuge tube comprising an upper cylindrical portion and a lower conical portion, a sterile tissue inlet fitting, at least one sterile processing fluid inlet fitting, a sterile suction fitting, and at least one sterile extraction port connected to a first extraction tube. The first centrifuge tube further includes an internal space including a screen being positioned therein, the screen being configured to divide the internal space in half, and a filter positioned therein, the filter being positioned below the screen in the lower conical portion of the first centrifuge tube. The device may further include a second centrifuge tube configured to receive and further process the biological substance from the first centrifuge tube. The second centrifuge tube has at least one sterile fitting, wherein the second centrifuge tube is releasably connected via the at least one sterile fitting to one of the at least one sterile extraction ports of the first centrifuge tube.

Some variations provide a process to produce renewable biogas and cellulosic fiber from a cellulose-rich feedstock, comprising: obtaining a cellulose-rich feedstock that is reduced in concentration of impurities and has a length less than 5 inches; feeding the cellulose-rich feedstock and water to a vacuum steam-explosion vessel, thereby generating a first cellulose-rich intermediate stream; feeding the cellulose-rich intermediate stream to a secondary separation vessel to remove remaining impurities, thereby generating a second cellulose-rich intermediate stream; feeding the second cellulose-rich intermediate stream to an enzymatic-hydrolysis vessel to convert the second cellulose-rich intermediate stream to low-viscosity cellulosic fibers comprising cellulose oligomers; feeding the low-viscosity cellulosic fibers to an anaerobic digester, thereby generating a biogas stream and residual fiber; optionally, recycling residual fiber to the enzymatic-hydrolysis vessel and/or to the anaerobic digester; and recovering some or all of the residual cellulosic fiber from the solid stream as a co-product.

Disclosed herein are devices and methods for processing biologic tissues, such as adipose tissue or whole blood, via centrifugation, An annular cavity is present, typically at the widest interior point of a rotatable chamber. The device is operable such that a sample of biologic tissue present in the rotatable chamber can be stratified into at least two constituent layers as a function of the differing specific gravities of the constituents and at least a portion of one of the constituent layers captured in the annular cavity.

The present application disclosed a cell centrifugation apparatus and a cell centrifugation, washing and culture method. The cell centrifugation apparatus comprises a centrifuge chamber (1) and a liquid pushing plate (2); the centrifuge chamber (1) has a rotation axis, and the centrifuge chamber (1) is used to connected with a rotation drive mechanism (4) and rotate around its rotation axis under drive of the rotation drive mechanism (4), so as to centrifuge a blood sample contained in the centrifuge chamber (1); and one or more liquid pushing plates (2) are arranged in the centrifuge chamber (1), when the centrifuge chamber (1) rotates, the liquid pushing plate (2) is used to push the blood sample to rotate synchronously along the same rotation direction as the centrifuge chamber (1).

A cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 13545 to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. In an embodiment, the device is used as a point-of-care and/or portable device. Further, the present disclosure describes software that, when executed by a processor, causes the device to perform the disclosed functions.

A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.

Blood from a blood source is drawn into a fluid flow circuit. A mononuclear cell product is separated from the blood, followed by at least a portion of the mononuclear cell product being conveyed into an electroporation device without disconnecting the blood source from the fluid flow circuit. The electroporation device opens pores in a membrane of at least one of the cells of the mononuclear cell product to allow DNA material (which is added to the mononuclear cell product prior to electroporation) to enter and modify the genome of the cell. At least a portion of the modified mononuclear cell product is returned to the blood source. The mononuclear cell product may be washed prior to being conveyed into the electroporation device. The modified mononuclear cell product may be washed after exiting the electroporation device.

The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant.

The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.