A fluid processing system includes a separation device, a mononuclear cell product processing device, a pump assembly, and a controller. The controller is programmed to actuate the pump assembly to convey blood from a blood source into the separation device, actuate the separation device to separate a mononuclear cell product from the blood, and actuate the pump assembly to convey at least a portion of the mononuclear cell product through the mononuclear cell product processing device to modify a genome of at least one of the cells of the mononuclear cell product and create a modified mononuclear cell product. The controller is programmed to selectively actuate the pump system to convey the mononuclear cell product through the mononuclear cell product processing device in either a first direction or in an opposite, second direction to modify the genome of at least one of the cells of the mononuclear cell product.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

The present invention efficiently and cost-effectively extracts and collects cells from a tissue. The inventors have discovered that the tissue can be effectively fragmented and the resulting cells can be purified using a system or kit with multiple components. An advantage of the present invention is that tissue processing takes place in a closed system such that sterility can be maintained throughout the process, even if certain components are removed during processing, for example through the use of valves, clamps, and heat seals. Furthermore, any or all of the steps can be automated or manually accomplished, according to the specific needs of the application or the user.

A system and method for automatically digesting, culturing and growing a biopsy. The digester is a closed fluid transfer system, including disposable and durable parts, that receives a biopsy, washes the biopsy tissue, digests the tissue to separate the cells from each other, and seeds the cells in a culture flask. In one use, the system receives cells from a patient, expands and multiplies them, and returns them to the patient. A controller coordinates the motion of a plurality of pumps and the status of a plurality of valves, the pumps and the plurality of valves moving the fluid throughout the system. A sensor system assists the controller in volume and flow rate control of the fluids in the system, and in controlling the position of a tube providing air and fluids to a biopsy vial that includes the biopsy.

Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceutical compositions comprising an enucleated cell.

A screen exchange device for reducing the size of a biological tissue according to one embodiment comprises: a disk comprising a plurality of screens each having at least one through-hole configured to reduce the size of a biological tissue, the screens having different through-hole characteristics; a first cover which covers a first side of the disk and includes a first opening through which a biological tissue passes; a second cover which covers a second side of the disk and includes a second opening through which the biological tissue passes; a housing configured to receive the disk; and a manipulator which selects any one screen among the plurality of screens and manipulates the first cover or the second cover such that the selected screen communicates with the first opening and the second opening.

The present invention is directed to a system, method, use, and diagnostic kit for cell-derived processing. The invention comprises a controlling component and a driving and/or heating component configured to host a cartridge and drive and/or heat the cartridge.

A cell modification system includes a separation module, a modification module, a culture module and a filter module. The separation module is configured to receive a blood sample, separate cells and plasma from the blood sample, and then separate target cells. The modification module is configured to receive the target cells and introduce a modification composite material into the target cells to convert the target cells into modified cells. The culture module is configured to receive and store the modified cells, and monitor a cell proliferation environment to facilitate the activation and proliferation of the modified cells. The filter module is configured to receive the modified cells after proliferating, determine whether the modified cells have been modified successfully, and output the cells that have been modified successfully as a therapeutic product. A cell modification device is also disclosed.

A portable apparatus useful for collection and processing of human biological material, such as adipose or cancellous bone material, to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a pellet well for collecting concentrate product in the form of a pellet phase from centrifuge processing. The pellet well is accessible only from above when the apparatus is in an access orientation. Collected pellet phase material may be removed from the pellet well by direct aspiration, without suspending the material in a suspension liquid within the container. Access ports may be configured for access only from above the container. The apparatus may include a tissue collector disposed in the disuse retention volume to engage and collect collagen or other stringy tissue. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.

Some variations provide a process to produce renewable biogas and cellulosic fiber from a cellulose-rich feedstock, comprising: obtaining a cellulose-rich feedstock that is reduced in concentration of impurities and has a length less than 5 inches; feeding the cellulose-rich feedstock and water to a vacuum steam-explosion vessel, thereby generating a first cellulose-rich intermediate stream; feeding the cellulose-rich intermediate stream to a secondary separation vessel to remove remaining impurities, thereby generating a second cellulose-rich intermediate stream; feeding the second cellulose-rich intermediate stream to an enzymatic-hydrolysis vessel to convert the second cellulose-rich intermediate stream to low-viscosity cellulosic fibers comprising cellulose oligomers; feeding the low-viscosity cellulosic fibers to an anaerobic digester, thereby generating a biogas stream and residual fiber; optionally, recycling residual fiber to the enzymatic-hydrolysis vessel and/or to the anaerobic digester; and recovering some or all of the residual cellulosic fiber from the solid stream as a co-product.