Methods and systems to isolate microvessels using an enriched or purified enzyme to dissociate tissue are described. The systems and methods include a second digestion to digest a top layer, from a first digestion and first centrifuge operation, with the enriched or purified enzyme to generate a second fat-enzyme solution, a second centrifuge operation, and isolation of the microvessels from pellets generated by the first and second centrifuge operations. The systems and methods may include washing the second fat-enzyme solution with an enzyme inhibitor in a post-digestion wash.

Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

Blood from a blood source is drawn into a fluid flow circuit. A mononuclear cell product is separated from the blood, followed by at least a portion of the mononuclear cell product being conveyed into an electroporation device without disconnecting the blood source from the fluid flow circuit. The electroporation device opens pores in a membrane of at least one of the cells of the mononuclear cell product to allow DNA material (which is added to the mononuclear cell product prior to electroporation) to enter and modify the genome of the cell. At least a portion of the modified mononuclear cell product is returned to the blood source. The mononuclear cell product may be washed prior to being conveyed into the electroporation device. The modified mononuclear cell product may be washed after exiting the electroporation device.

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

A device for processing a liquid sample containing cells is provided. The device includes a stationary part and a rotatable part. The stationary part includes a cap, a socket connected to the cap, and a tube located within the cap and the socket. The rotatable part includes a centrifugal processing chamber, and a flow guide located within the centrifugal processing chamber. The stationary part and the rotatable part are connected by a plurality of rotary dynamic seals allowing rotation of the rotatable part relative to the stationary part. The cap includes a first port connected to an inlet, and a second port connected to an outlet. The centrifugal processing chamber includes an upper wall, a neck extending from the upper wall, a sidewall and a bottom wall tilting downwardly.

The present invention relates to a device and a method for FIG. 2 treating fat cells taken from a patient and intended for a transplant. More specifically, it relates to a device and a method by suctioning which makes it possible to separate the viable fat cells from the residues and elements which are not transplantable. The device is a device for treating adipose tissues (G) connected to a suctioning means (M), consisting of a sealed treatment chamber (A) comprising a means for connection (17) to said suctioning means (M), a means for inlet (13) of said adipose tissues before treatment, a filtration means (3) and at least one means for outlet (20) of said adipose tissues (G) after treatment, said filtration means (3) comprising a conically shaped external wall (3 1, 3) comprising a narrow base (55) attached to an impeller (25) and an internal sieving means (30) connected to the output means (20), said impeller (25) being subjected to the air pressure reduction created by the suctioning means (M) and constituting a rotary mechanical actuating means for actuating said filtration means (3).

An automatic cell isolation and collection system for sorting out target cells from a sample includes a crush module, a centrifuge module, a transport module, and a control unit. The centrifuge module includes centrifuge tubes and a magnetic mechanism providing a magnetically attractive force to at least one of the centrifuge tubes. The control unit electrically communicates with the crush module, the centrifuge module, and the transport module, and controls operation of the crush module, transport of the sample via the transport module after the sample is crushed by the crush module, centrifugation of the centrifuge module, and operation of the magnetic mechanism for providing the magnetically attractive force.

The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.

A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.