An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for grafting to a patient. In applying the method and/or in using the device, donor tissue is harvested, subjected to a cell dissociation treatment, cells suitable for grafting back to a patient are collected and dispersed in a solution that is suitable for immediate dispersion over the recipient graft site.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.

An isolation device for adipose-derived stromal vascular fraction is provided. More particularly the embodiments relates to novel systems, devices, methods and kits for adipose tissue collection and stromal vascular fractions isolation from collected adipose. The devices and systems are used in the field of healthcare/regenerative medicine.

Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.

The invention relates to a method for treating biomass (2). Biomass (2) is fed to a pressurized prehydrolysis reactor unit (8) by means of a feeding system (5, 7), wherein by means of the feeding system (5, 7) the biomass (2) is compressed. A filtrate is squeezed out of the biomass (2) by means of the feeding system (5, 7), in particular by a first plug screw (5) or a second plug screw (7) of the feeding system (5, 7). The biomass (2) is then thermally treated in the pressurized prehydrolysis reactor unit (8), discharged from the pressurized prehydrolysis reactor unit (8) afterwards, diluted with the filtrate before or after the discharge, and treated with an enzyme subsequently.

A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185° C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process.

The invention concerns a method for treating lignocellulosic biomass, said method comprising the following steps: a, Preparing an impregnation liquor (4) containing a chemical catalyst intended to impregnate the biomass b. Introducing the crushed biomass (6) through an inlet of an impregnation reactor (5), said inlet being situated in a first impregnation area (5a) of said impregnation reactor that comprises two superposed areas, said first impregnation area and a second so-called dewatering area (5b) above the impregnation area c. Introducing the liquor (4a) through a first liquor inlet situated in said first impregnation area (5a) of the reactor, d. Introducing said liquor (4b) into said reactor through a second liquor inlet in another area of the reactor (5d) situated below the biomass inlet in the first impregnation area (5b). The invention also concerns the facility for implementing the method.