The invention relates to a method and a biogas plant for producing biogas, preferably from rice straw, wherein a substrate is fermented in two reactors (1, 2) in a circulating manner, so that a methane production from cellulose-and/or lignocellulose-containing substrate can be improved.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues and in some embodiments use the single cells to form organoids or microtissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells and then use a hanging droplet method to form organoids or microtissues.

Provided is an electric perforating device 10 including a flow passage 20 through which liquid flows, a first electrode 30A that forms a first electric field region 60A in the flow passage 20 by being supplied with a pulse voltage, a second electrode 30B that forms a second electric field region 60B on a downstream side of the first electric field region 60A, which is in the flow passage 20, in a flowing direction of the liquid, by being supplied with a pulse voltage, a cooling portion 40 that forms a cooling region 70, which cools the liquid flowing through the flow passage 20, between the first electric field region 60A and the second electric field region 60B in the flow passage 20, and a voltage output portion 50 that outputs a pulse voltage for forming the first electric field region 60A and the second electric field region 60B.

The automatic bearing device for living cell freezing and thawing includes a droplet changing area on one end and a sample bearing area communicating with the droplet changing area and arranged on the other end. The droplet changing area has a structure of a shallow dish for droplet change, and the bottom surface gradually inclines downwards from the free end to one end communicating with the sample bearing area. The sample bearing area has an elongated concave dish for containing frozen/thawed carriers. At least one part of the side of the droplet changing area is connected with the corresponding side of the sample bearing area. A sealer is arranged in the connection position, and a removable baffle is arranged above the connection position. The device realizes rapid and effective vitrification for living cell freezing and thawing.

The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurized pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurized pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

The present invention provides a method for recovery of phosphate, in the form of magnesium ammonium phosphate (MAP), from a process for treating a biomass material which process comprises a digestion step performed in a digestion tank and includes a pre-treatment step employing a thermal hydrolysis, characterized in that a magnesium source is added to the material in the process flow before said flow enters the digestion tank, and phosphate is recovered as MAP as an integral part of a solid or semi-solid digestate product from the digestion tank.

An interface for coupling a dissolved oxygen sensor to a single-use bioreactor container is provided. A dissolved oxygen (DO) window membrane is operably coupled to the single-use container and configured to position a DO sensor at least partially within the single-use container. In some embodiments, a DO window body mounts the DO window membrane at a distal end thereof. The DO window body can include a slide lock for facilitating positioning of a DO sensor within the DO window body. Additionally, the DO window body may include at least one heat exchange fin.