Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.

The present invention relates to a packaging method for increasing the shelf life of fungal spores and to the packaging that contains such spores. The packaging method comprises the steps of pre-drying the spores to a water activity range viable for the fungus of interest, followed by packing of the spores into gas- and water-vapor-impermeable packaging by means of the use of sachets, thereby providing an atmosphere of low relative humidity and low oxygen content, followed by keeping of the spores in the packaging for a certain period of time at the temperature suitable for the spores prior to the exposure thereof to high temperatures.

The carriers that are available in the market don't maintain all the necessary parameters that are mentioned in the subject matter of this application. Many of them are: 1) Costly 2) Allow the transport of cells for short period of time 3) Are suitable for transporting certain type of cells 4) Don't include control for all the parameters that are required for the experiments 5) Need extra steps that can affect cell/organs and waste the time, efforts and money of the researchers. 6) Limit researchers for using containers with certain sizes and materials. 7) Involve changing the conditions of cell growth such as splitting the cells, freezing, centrifuge them during the process of transporting them. Alternatively, the Mini-incubator carrier box in my invention is an economic transport system and practical. It enables the researchers to fix different containers at different sizes using clamps and screws and to transport the organs/samples/cells in any container for long period of time and long distance under optimized conditions without the need of extra steps/costs.

In some embodiments, a kit for preparing a cell suspension may include a device and a housing. The device may include a first label identifying a first reservoir of the device for use with a first portion of a tissue processing method and a second label identifying a second reservoir of the device for use with a second portion of the tissue processing method. The housing includes a first housing portion configured to store a first set of components associated with the first portion of the tissue processing method. The first housing portion includes a first visual indicator associated with the first label of the device. The second housing portion may be configured to store a second set of components associated with the second portion of the tissue processing method. The second housing portion may include a second visual indicator associated with the second label of the device.

The current disclosure provides a method for the creation of a high-density cryopreserved cell bank using perfusion culture techniques and non-centrifugal concentration of cells. Methods of production using this high-density cryopreserved cell bank are also provided.

A culture container transportation set suitable for cultured state-maintaining transportation is provided. A culture container transportation set A1 includes: a culture container 13 including a vessel 13 made up of a bottom wall 11 and a tubular side wall 12 rising from the bottom wall 11; a flexible cover 2 covering an upper edge portion 121 of the side wall 12; a hard pressing member 3 provided on the cover 2; a cushioning material 4 of shape restorability; and a housing container 6 that houses the culture container 1, the cover 2, the pressing member 3 and the cushioning material 4 in an assembled state in which these components are stacked while pressing these components from above and below. The cushioning material 4 is provided between the housing container 6 and the culture container 1 or between the pressing member 3 and the housing container 6.

Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.

The invention provides a cell and biotissue transporting container for transporting cells or biotissues while maintaining their cultured state. A cell/biotissue transporting container A10 includes a base member 1 with an opening 110 at an upper end and a first tubular portion 11 extending vertically, a lid body 2 including a top plate portion 21 to close the opening 110 and a second tubular portion 22 extending from a peripheral edge of the top plate portion 21 in the thickness direction to fit onto the first tubular portion 11, and a container body 3, formed separately from the base member 1 using a flexible material, where the container body includes a bottom wall portion 31, a tubular side wall portion 32 rising from a peripheral edge of the bottom wall portion 31 and inserted into the opening 110, and a flange 33 extending outward from an upper edge of the side wall portion 32. At least one of the first and second tubular portions 11 and 22 includes a lock preventing relative movement between the base member 1 and the lid body 2 with the flange 33 being clamped between a peripheral edge portion of the opening 110 of the base member and the top plate portion 21 of the lid body 2.

The present invention generally relates to the use of porous polymer materials as a medium for the storage of biological samples. The present invention also relates to a method of drying and storage of biological samples on the porous polymer materials. The biological samples include blood and blood plasma samples.

Cryogenic devices are provided in which solid carbon dioxide (dry ice) is used to maintain a temperature zone in which samples can be manipulated under conditions in which the sample is maintained at a temperature below 50 C.