A media tray system may include a tray having a base wall and a perimeter wall extending upwardly from the base wall and having an upper edge. The system may also include a cover having a top wall and a side wall extending downwardly from the top wall to extend about the perimeter wall of the tray when the cover is in a closed condition with respect to the tray, with the side wall having a lower edge. The system may further include a cover support structure located at the first end of the tray and the first end of the first end of the cover. The cover support structure may be configured to support the cover in an access condition permitting access to the tray interior of the tray through a gap formed between an upper edge of the tray and the lower edge of the cover.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A test tube, suitable to be immersed in a cryogenic fluid, comprises a containing body having a perimeter wall that defines a containing compartment, a bottom wall, disposed inside the latter to close it at the lower part, and a housing seating, inside the perimeter wall and outside the containing compartment. A support element for an identification code is suitable to be inserted into the housing seating, with at least a free surface facing toward the outside of the housing seating on the opposite side with respect to the bottom wall. In correspondence to said housing seating, mechanical constraint means are provided in cooperation with said perimeter wall of the containing body and with a peripheral portion of the free surface of the support element, in order to constrain the latter in a defined axial position with respect to the containing body.

This invention relates to a method of detecting a gene. The invention also relates to a method of determining the Amplification expression level of a gene. The invention also relates to compositions for use in these methods.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

Disclosed herein are standardized and optimized, modular single-use kits and methods for sterile or non-sterile preparation of biological material, such as cells or tissue, for immediate use, further preparation, or storage. These single-use kits comprise at least one single-use component necessary for the preparation of biological material in accordance with a particular method as set forth in the instructions for use included with each particular type of single-use kit that set forth a unique method for using that particular type of single-use kit. The single-use kit of the invention comprises at least one modular, single-use packaging frame wherein at least one modular, single-use tray with an identifying is disposed, wherein at least one single-use component is organized. The identifying label on each modular single-use tray corresponds to (correlates with) a sequence of steps set forth in the instructions included with each single-use kit. The single-use kits of the invention facilitate and improve a healthcare practitioner's ability to prepare biological material for research, diagnostic and therapeutic purposes in a convenient, consistent, repeatable, safe and effective manner.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

A method of preparing a fat graft includes introducing fat tissue into an internal chamber of a vessel of a cell or tissue isolation, purification, and storage device through a first opening of the vessel. The method also includes introducing an amount of a wash solution effective to wash the fat graft or an amount of a cryoprotectant effective to cryopreserve the fat graft into the internal chamber of the vessel through a second opening of the vessel and mixing the fat tissue with the wash solution or the cryoprotectant. The method further includes either: when wash solution is introduced, centrifuging the device containing the fat graft to separate the fat graft from the wash solution and drawing the wash solution from the first end of the device; or, when cryoprotectant is introduced, cooling the fat graft to a temperature below 0? C.