Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.

The present disclosure relates to settling devices for separating particles from a bulk fluid with applications in numerous fields. The particle settling devices of the present disclosure may include a stack of truncoconical cones that may be arranged in opposite orientation, apex to base. Other embodiments include several concentric vertical tubes attached to conical surfaces at the bottom, with inclined settling strips attached to the vertical tubes in annular regions between the tubes. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. The nutrient fluid stream dislodges some cells from the cell culture, which can then be recovered for cell therapy applications. The cell culture continues to expand and reproduce, permitting continuous recovery of cells from the bioreactor.

A device (1) and a method are provided for treating a porous filtration medium (37) having a receiving unit (2) with of a receiving part (5) and a base part (6). The porous filtration medium (37) can be lifted by the receiving part (5) from a lower part (33) of a filtration device (32), and the receiving part (5) with the porous filtration medium (37) can be mounted on the base part (6). The receiving part (5) is latchable to the base part (6). The base part (6), towards the filtration medium (37) has an incubation chamber (17) connected to a base part (6) outlet (3) that faces away from the receiving part (5), and the outlet (3) has a projection onto which a receiving vessel (4) containing a solvent (28) for dissolving the porous filtration medium (37) can be detachably pushed on.

A tissue processing apparatus, a filter and a method for processing tissue therefrom #! The problem to be solved is to provide a portable apparatus for processing harvested fat to form a good quality graft that does not clog syringes, and the problem is solved by providing an apparatus as in the present invention with a filter that is inclined to the base of the apparatus and comprises of plurality of elongated protrusion that are structured at an angle on the surface of the filter and blocks the fibres when the harvested fat moves on the inclined surface of the filter, thereby filtering the harvested fat and avoiding clogging of syringes.

A method for separating a biological suspension includes dispensing a liquid suspension comprised of cells or microorganisms from a bioreactor or fermenter into a sterile compartment of a first bag assembly, the first bag assembly including a collapsible bag having of one or more sheets of flexible film. The compartment of the first bag assembly is sealed closed. The first bag assembly, either with or without a manifold fluid coupled therewith, is then rotated, such as by using a centrifuge, so that the liquid suspension separates within the compartment into a pellet comprised of the cells or microorganisms and a liquid supernatant.

A microfluidic system for cell retention for a perfusion bioreactor is provided. The system comprises at least one inlet configured to receive a bioreaction mixture to be processed. At least one curvilinear microchannel is in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate cells in the bioreaction mixture, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel. At least two outlets are in fluid flow connection with the at least one curvilinear microchannel. At least one outlet of the at least two outlets is configured to flow the isolated cells to be recycled to the perfusion bioreactor.

An object of the present invention is to provide: an algae water concentration system that efficiently concentrates algae water in a culture pond into algae water containing algae having a desired size, with a simple structure and at low cost; and a method for operating the same. A cultured algae water concentration system 100 comprises: an algae water supply unit 17 that has an algae water supply container 18 that stores algae water sent from the culture pond therein, and a supply container outlet port 19 through which the algae water is taken out from the algae water supply container; and an algae water concentration unit that has a concentration container 1 for receiving and concentrating the algae water from the algae water supply unit, a filter 3 which divides the concentration container into upper and lower spaces and does not pass algae having a predetermined size or larger therethrough, a vibration device 5 that vibrates the filter in an out-of-plane direction, a concentration container algae water inlet port 7 which takes in the algae water to the concentration container and is arranged below the filter of the concentration container, a concentrated algae water outlet port 9 that is arranged below the filter of the concentration container and takes out algae water therethrough that has been concentrated in the concentration container, and a filtered water discharge port 8 that is arranged above the filter of the concentration container and discharges filtered water having passed through the filter.

Aspects of the disclosure are directed to an apparatus for separating a second fluid or a particulate from a host fluid. That apparatus comprises a flow chamber with at least one inlet and at least one outlet. A drive circuit configured to provide a drive signal to a filter circuit configured to receive the drive signal and provide a translated drive signal. An ultrasonic transducer is cooperatively arranged with the flow chamber, and transducer includes at least one piezoelectric element configured to be driven by the current drive signal to create an acoustic standing wave in the flow chamber. At least one reflector opposing the ultrasonic transducer to reflect acoustic energy.