Improved processes and systems for recovering products from a corn fermentation mash. In some examples, a process recovers an oil product, a protein meal product, and a fiber product from a slurry. A process includes the following steps: introducing the slurry into a device with a flexible screen and mechanical agitation to produce a filtrate and a fibrous solid stream; and introducing the filtrate into a three-phase centrifuge to produce an oil stream, a high protein solids stream, and a water with solubles stream. The flexible screen includes a washing nozzle. The slurry is whole stillage from an ethanol process. The three-phase centrifuge is a three-phase decanter. The filtrate is heated before introducing into the three-phase centrifuge. The filtrate is optionally evaporated before introducing into the three-phase centrifuge.

Provided is an automated system for preparing purified plasmid DNA in commercial scale.

An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

A method and microfluidic device for increasing the production of extracellular vesicles are provided which include the use of a cell-adhesive substrate for culturing extracellular-vesicle-producing cells, wherein the cell-adhesive substrate is a hydrogel having a Young's modulus of about 0.1 kPa to about 5 kPa and the hydrogel is functionalized with one or more cell adhesive peptides.

Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be trans-located. The exemplified unfoldase is the ClpX unfoldase from E. coli.

Embodiments disclose an apparatus and methods for biological sample processing enabling isolation and enrichment of microbial or pathogenic constituents from the sample. A vessel for sample containment and extraction is further disclosed for engagement with a transducer capable of efficient sample disruption and lysis. Together these components provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.

A system and a method for treating biomass with a gas includes at least one conduit having at least one biomass inlet and at least one biomass outlet, at least one gas inlet and at least one gas outlet. The system further includes a transport unit configured to move the biomass through the conduit from the at least one biomass inlet to the at least one biomass outlet thereby defining a biomass transport direction. The system is configured such that gas flowing from the at least one gas inlet to the at least one gas outlet crosses the biomass transport direction.

An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.

The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit, wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates increasing protein product yield from bacterial cells are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.