C12M47/06

Processes for separating whole stillage to produce a fiber rich product that includes C5 and C6 polysaccharides
11254955 · 2022-02-22 · ·

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product.

Containers for agitation of liquid samples and methods of use thereof

The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.

Device and method for treating a filtration medium

A device (1) and a method are provided for treating a porous filtration medium (37) having a receiving unit (2) with of a receiving part (5) and a base part (6). The porous filtration medium (37) can be lifted by the receiving part (5) from a lower part (33) of a filtration device (32), and the receiving part (5) with the porous filtration medium (37) can be mounted on the base part (6). The receiving part (5) is latchable to the base part (6). The base part (6), towards the filtration medium (37) has an incubation chamber (17) connected to a base part (6) outlet (3) that faces away from the receiving part (5), and the outlet (3) has a projection onto which a receiving vessel (4) containing a solvent (28) for dissolving the porous filtration medium (37) can be detachably pushed on.

METHOD FOR CULTIVATING A MICROORGANISM OF INTEREST AND ASSOCIATED FACILITY
20220049211 · 2022-02-17 ·

A method for cultivating at least one microorganism of interest, by heterotrophism or mixotrophism, in an aqueous culture medium, contaminating microorganisms developing naturally in the culture medium. A portion of the culture medium with the microorganism of interest and the contaminating microorganisms is sampled. The microorganism of interest and the contaminating microorganisms in the portion of culture medium is physically separated. The contaminating microorganisms thus separated is lysed to produce a lysate. The lysate is reintroduced into the culture medium.

COUPLED SORTING AND ELECTRIC TREATMENT OF BIOLOGICAL CELLS
20220049208 · 2022-02-17 ·

The present application provides a method for lysis or electroporation of cells in a biological sample including the following steps: passing cells of the sample, suspended in a fluid, through a flow path with a preset flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path includes at least two electrodes for generating an electric field, which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coaling at least covers the surface of the electrodes that faces the flow path, and when the presence of a specific cell is detected in the detection apparatus, then an electric field is generated between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the electric field causes electroporation or lysis of the cell.

SPECIFIC ELECTROPORATION AND LYSIS OF EUKARYOTIC CELLS
20220049239 · 2022-02-17 ·

A method of targeted electroporation and/or lysis of eukaryotic cellular bodies in a biological sample with at least two subgroups of eukaryotic cellular bodies, wherein each subgroup has a different susceptibility to electroporation and/or lysis in electric fields, including the following steps: transferring the biological sample in a chamber, exposing the biological sample to an electric field in the chamber, wherein the electric field is generated by at least two electrodes which are coated with a dielectric material with a relative permittivity greater than 3.9, and selecting the electric parameters of the electric field such as the field strength, the frequency or the wave form so that the subgroups are differently affected by said electric field for electroporation and/or lysis; as well as devices for the method.

DEVICE FOR NUCLEIC ACID EXTRACTION USING MAGNETIC BEAD METHOD
20170275614 · 2017-09-28 ·

The present invention provides a device for nucleic acid extraction using a magnetic bead method. The device includes a disc turntable, an arc arch bridge base, a supporting column, and a magnet structural unit and a sample reaction unit both arranged on the disc turntable. The disc turntable includes a top plate; the arc arch bridge base includes a bottom plate arranged at the lowermost portion and an arc supporting plate arranged on the bottom plate, and the arc supporting plate corresponds to the guide rod insertion holes in position in a radial direction of the disc, and the arc supporting plate includes raised regions used for jacking up a guide rod and a magnet and groove regions used for allowing the guide rod and the magnet to fall. The device provided by the present invention can realize the automatic nucleic acid extraction in a stream-lining manner.

SYSTEM AND METHOD FOR PROCESSING BIOMASS
20170275583 · 2017-09-28 · ·

System (100) and method for processing biomass. The system comprises a combined heat and power plant (102), an interface (114) for feeding biogas to a traffic fuel production unit, interfaces (114) to a district heating system (106a) and an electrical grid (106b), and a hydrolysis device (108), a digestion device (110), a dryer (116) and a heat recovery unit (112), which are operatively coupled for transferring heat, intermediate products and final products of the process, wherein raw biomass is received into the Fuel hydrolysis device (108), biomass processed by the hydrolysis device (108) is fed to the digestion device (110), biogas obtained in the digestion device (110) is fed to the traffic fuel production unit (104), heat is recovered from the hydrolysis device (108), biomass processed by the digestion device (110) is dried by the heat recovered from the hydrolysis device (108), heat is recovered from the dryer (116), heat recovered from the dryer (116) is fed to the hydrolysis device (108) to be used in pre-heating of the received raw biomass, heat recovered from the dryer (116) is fed to the district heating (106a), and production of electricity is fueled by the dried biomass from the dryer (116).

APPARATUSES, METHODS AND SYSTEMS FOR AUTOMATED PROCESSING OF NUCLEIC ACIDS AND ELECTROPHORETIC SAMPLE PREPARATION

Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis
11427854 · 2022-08-30 · ·

A method for collecting microorganisms when contained in a fluid includes (i) introducing the fluid into a cavity of a collecting device via at least one admission duct, (ii) capturing the microorganisms when contained in the fluid with a set of beads retained in the cavity as the fluid passes through the set of beads, (iii) evacuating the fluid from the cavity via at least one evacuating duct, (iv) introducing a reaction liquid into the cavity via at least one admission channel, (v) collecting the microorganisms from the set of beads with the reaction liquid as the reaction liquid passes through the set of beads, and (vi) evacuating the reaction liquid from the cavity via at least one evacuating channel.