A method of producing a cell growth medium. The method includes cultivating microbial cells by gas fermentation to obtain a biomass slurry, concentrating the biomass slurry by separating and removing a liquid phase from a solid phase, homogenizing the concentrated biomass slurry with high pressure homogenization, wherein the microbial cells are at least partially degrading, treating the homogenized biomass slurry by adding at least one protease, and heating the treated biomass slurry.

Reference is made to the Identification of the Microorganism, with the identification reference given by the DEPOSITOR of SoF1, having the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY of VTT E-193585, received on Jun. 11, 2019, (date of original deposit).

A method for producing astaxanthin incorporates a method for producing astaxanthin-rich algae cells and a method for extracting astaxanthin therefrom. An initial feedstock comprises healthy algae, water, and nutrients. During a growth phase, carbon dioxide and light from a light source are supplied to the feedstock, thereby amplifying the algae. At least a portion of the nutrients remaining after amplification of the algae are separated from the amplified algae. During a stress phase, carbon dioxide and light are supplied to the amplified algae, thereby promoting production of astaxanthin by the amplified algae. The amplified algae and a cover are placed within an interior of an attrition mill having interior surfaces and media which are substantially non-reactive to astaxanthin and milled to release the astaxanthin from the algae. The cover limits oxidation of the released astaxanthin.

A device and method for lysing biological species present in a fluid includes implementing a rough surface against which the objects to be lysed are crushed by a shearing motion. A device for mechanically lysing biological species has a first and second wall mounted movable relative to each other, between an initial position in which the walls are separated from each other, and a lysing position in which the first wall presses against the second wall. The first and second walls also are mounted shearingly movable relative to each other in the lysing position. At least one of the first or second walls has a rough bearing surface against the other wall and has a mean surface roughness parameter Ra of between 0.2 m and 10 m, and preferably between 0.2 m and 3 m.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates increasing protein product yield from bacterial cells are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

A method for producing a plant life enhancing product is described. The method includes: (i) anaerobically digesting agricultural residue to produce an anaerobically activated organic residue and an ammonia-enriched water; (ii) aerobically digesting the ammonia-enriched water to produce an aerobically activated organic residue; and (iii) blending the anaerobically activated organic residue with the aerobically activated organic residue to produce the plant life enhancing product.

The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 m to 300 m; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

This invention relates to a continuous flow homogenization device and method of using the device for lysis or disruption of cells, tissues, and other biologicals and creating smaller particles and emulsions. The device contains a homogenization chamber in which incoming material continuously enters the chamber via an inlet and the homogenized material continuously flows out via an outlet. The homogenization chamber may include at least one impeller that rotates and/or reciprocates inside the chamber in the presence or absence of a homogenization enhancement media. The homogenization enhancement media may include rigid particles. All product contact surfaces can be sterile and disposable. The complete homogenization process can be executed in a fully automated or manual manner by a controller that manages different inputs and outputs of the system components.

Disclosed is an ultrahigh-pressure homogenizing integrated device and a cell disruptor. The ultrahigh-pressure homogenizing integrated device includes a long oil cylinder, a main connecting sleeve, a high-pressure cylinder homogenizing main body, an auxiliary connecting sleeve and a short oil cylinder, which are sequentially and coaxially arranged. An upper part of the high-pressure cylinder homogenizing main body is provided with a feeding hole communicated with a high-pressure cavity; and the feeding hole is connected with an integrated feeding device. A pressurizing plunger rod in the high-pressure cavity of the high-pressure cylinder homogenizing main body is connected with a piston rod of the long oil cylinder; and a homogenizing valve arranged in the inner cavity, which is communicated with the high-pressure cavity, of the high-pressure cylinder homogenizing main body, is connected with an ejector rod of the short oil cylinder.

A method for processing raw liquid manure and/or fermentation residues involves providing raw liquid manure and/or fermentation residues, purifying the raw liquid manure and/or fermentation residues in a first purification stage so as to form a first solid phase and a first purified liquid phase, pre-conditioning the first purified liquid phase in order to separate ultrafine particles from the first purified liquid phase, and purifying the first purified liquid phase in a second purification stage so as to form a second solid phase and a second purified liquid phase.