A multiple-loop tangential flow filtration apparatus for concentrating fluids is described herein. The apparatus comprises a plurality of tube loops for receiving fluid therethrough, each tube loop comprising a respective filter, and a common feed pump for driving the fluid across each respective filter. The plurality of tube loops are coupled to the common pump via a common feed line.

Methods, systems and apparatuses herein provide a unique and novel process to process poultry litter. In one embodiment, the poultry litter is anaerobically digested to produce a biogas and one or more nutrients. In one embodiment, the poultry litter is wettened with recycled digestate prior to anaerobic digestion.

An apparatus and a method for crystallization and supercritical drying of culture solution are provided.

According to the embodiments of the present invention, an apparatus for crystallization and supercritical drying of culture solution to obtain target material by drying a culture solution containing a first solvent and the target material dissolved in the first solvent comprises a high pressure container to accommodate the culture solution, a first supply unit connected to the high pressure container to supply a second solvent to the high pressure container, a second supply unit connected to the high pressure container to supply a third solvent to the high pressure container, a precooler disposed adjacent to the first supply unit to precool the second solvent discharged from the first supply unit, and a preheater disposed adjacent to the high pressure container to preheat the second solvent and the third solvent supplied to the high pressure container.

According to the embodiments of the present invention, a method for crystallization and supercritical drying of culture solution to obtain target material by drying a culture solution containing a first solvent and the target material dissolved in the first solvent comprises crystallizing the target material by replacing the first solvent with a second solvent in the culture solution, and changing a phase of the second solvent to a supercritical phase.

A trans-disciplinary system for cell-free biosynthesis includes a cell-free transcription-translation (TX-TL) tool and modular, generalizable microfluidic architectures. Both components of the system are independently functional and are combinable into a cell-free biosynthesis platform. In the first component, modular plasmid libraries are used to program bacterial cell-free TX-TL systems. Each plasmid holds one gene or operon, and all the genes are controlled by the same promoter, so that the stoichiometry of enzyme synthesis is determined by the stoichiometry of plasmids in the reaction. In the second part, in order to facilitate high throughput mixing and matching of gene units from the modular plasmid libraries, a modular, reconfigurable, flexible, and scalable microfluidic architecture is employed. The microfluidic modules share common form factors and port/valve locations, so that a small set of module types, with multiple instances of each type interconnected in different geometries, allows simple reconfiguration to achieve different modes of operation.

Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product.

An extra-capillary fluid cycling unit for maintaining and cycling fluid volumes in a cell culture chamber includes a housing and a first flexible reservoir extra-capillary fluid reservoir disposed in the housing. The extra-capillary fluid reservoir is in fluid communication with a cell culture chamber. A second flexible reservoir is also located in the housing, the second flexible reservoir being in fluid communication with a pressure source. A sensor plate is movably disposed in the housing between the extra-capillary reservoir and the second reservoir, wherein the second reservoir is pressurized to move the sensor plate in relation to the extra-capillary reservoir to cause fluid cycling and maintain fluid volumes in the cell growth chamber.

A fluidic system for loading a therapeutic or imaging agent into the lumen of extracellular vesicles from producer cells, including at least one container, a liquid medium contained in the container, producer cells, a liquid medium stirrer and a device for controlling the speed of the stirrer suitable for the growth of the producer cells, wherein it also includes a device for controlling the speed of the stirrer and the stirrer, of which the shape and dimensions of the container are suitable for generating a turbulent flow of the liquid medium in the container for exerting shear stresses on the producer cells in order to carry out the loading of a therapeutic or imaging agent into the lumen of the extracellular vesicles produced simultaneously by the fluidic system.

The invention relates to a biogas plant for fermenting organic materials and for generating biogas, having a plurality of containers and/or tanks, which form at least one slurry store (7), at least one fermenter container (8) downstream of the slurry store (7), at least one post-fermenter container (9) downstream of the fermenter container (8) and/or at least one final storage container (10) downstream of the fermenter container (8). According to the invention it is provided that the entire functional biogas plant is, as a floating biogas plant, a component of a one-part or of a multi-part connected floating body.

Integrated systems and methods for the manufacturing of recombinant proteins in mammalian cells are provided. The systems and methods include a single-use bioreactor (SUB), a tangential flow filtration system, a column chromatography skid, a tankless hold, a virus inactivation plug flow reactor, and pumps.

Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.