The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and/or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

A gas permeable cell culture device including a container body defining an interior volume, the container body having a semi-permeable membrane defining one end of the interior volume, a fitting defining an opposing end of the interior volume, and a cell transfer conduit. The cell transfer conduit having an open end positioned within the interior volume between the semi-permeable membrane and the fitting and a radial portion disposed within the interior volume and between the open end and opposing end of the container body.

An automated isolation device for isolation of mitochondria includes an incubation station including a holder for a viable mitochondria solution; and a cooling system, controlled by a processor of the device, for cooling the holder. The device includes a processor-controlled transfer system for transferring solution from the holder to a filtration station; and the filtration station, including a series of filters. The device includes a processor-controlled spectrometry station including a spectrometer positioned to illuminate a cuvette fluidically coupled to an output of the filtration station; and a detector coupled to the processor and positioned on a side of the cuvette opposite the spectrometer. The device includes a processor-controlled transfer system for transferring solution from the spectrometry station to a centrifuge. The centrifuge is processor-controlled and configured to centrifuge the filtrate to separate viable mitochondria from a supernatant.

A cell printing apparatus of the present disclosure comprises a nozzle configured to discharge a heat-sensitive cell printing composition; and a heating unit for transferring heat to the upper side of the heat-sensitive cell printing composition which is discharged from the nozzle to be laminated. A predetermined space is formed between the heating unit and the nozzle so that the heating unit does not contact with the nozzle.

The present disclosure features methods and compositions for increasing the amount of products of cellular metabolism, e.g., proteins, by lowering the temperature of cells expressing the product at one or more steps while culturing the cells, expressing the product, and/or recovering the product.

A facility for the recycling of biomaterial with a fermentation stage, whereby the fermentation stage has a fermentation chamber for the production of biogas through anaerobic fermentation of the biomaterial, and with a hygiene stage that is positioned downstream from the fermentation stage, whereby the hygiene stage has a hygienization chamber for the reception and the thermal hygienization of biomaterial discharged from the fermentation stage. A process for the recycling of biomaterial by zymosis is also provided, whereby biogas is produced in a fermentation stage through anaerobic fermentation of the biomaterial, whereby the biomaterial is, after flowing through the fermentation stage, conveyed to a hygiene stage in which the biomaterial thermal is hygienized, and whereby the biomaterial is, after flowing through the hygiene stage, made available as recyclable agricultural, hygienized fermentation residue.