Patent classifications
C12N1/005
METHOD FOR PREPARING PURE PLANT-BASED MICROBIAL CULTURE
The present invention relates to a method for preparing a pure plant-based microbial culture, and provides: an application of a culture technology using both plant-derived juice and dietary fiber materials; and a culture and processing process for processing plant-derived vitamins and functional polysaccharides in a usable form, and adsorbs a microbial culture on a pure plant-based dietary fiber so as to maximally inhibit use in the oral cavity and digestive organs by harmful bacteria and selectively utilize microbial culture for the growth of beneficial bacteria, and thus, the present invention forms beneficial bacteria-oriented microcosms by strongly inducing the selective growth of beneficial bacteria preferentially in microcosms in the oral cavity and digestive organs.
ARTIFICIAL RECEPTORS, RECOMBINANT CELLS COMPRISING THEREOF, METHODS FOR THEIR PREPARATION, AND METHOD OF USING THEREOF
The disclosure presented herein provides artificial receptors and a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.
Composite particles, composite particles for forming liquid-encapsulating particles, liquid-encapsulating particles, method for producing liquid-encapsulating particles, biocatalyst-containing material, biocatalyst-containing material producing apparatus, and biocatalyst-containing material producing method
Provided are composite particles including hydrophobic solid particle A and hydrophobic solid particle B over surface of hydrophobic solid particle A, wherein contact angle CAa of hydrophobic solid particle A with water is 110 degrees≤CAa≤180 degrees, contact angle CAb of hydrophobic solid particle B with water is 110 degrees≤CAb≤180 degrees, ratio (d50a/d50b) of number average particle diameter d50a of hydrophobic solid particle A to number average particle diameter d50b of hydrophobic solid particle B is 10≤(d50a/d50b)≤100, and coating ratio CR of composite particles expressed by Formula 1 is 50%≤CR≤500%,
CELL CULTURE APPARATUS AND MEDIUM EXCHANGE METHOD
By swinging a culture vessel before aspiration removal of a medium, a centrally dense state (83) of the cell population is formed. In the centrally dense state (83), a cell cluster (84) is separated from an outlet port (58a). After aspiration removal of the medium, a new medium is introduced into the culture vessel. After the introduction of the new medium, an overall dispersed state of the cell population is formed.
Pasteurisation process for microbial cells and microbial oil
An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least −0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, one obtains a trapezium having an area less than 13,000° C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidised) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.
DRIED BACTERIAL CELL POWDER CONTAINING A CAROTENOID AND METHOD FOR PRODUCING THE SAME
According to the present invention, a powder containing a carotenoid for feed having improved color enhancing ability, and a method for producing the same are provided. A method for producing a dried bacterial cell powder containing a carotenoid, comprising a step of drying via conductive heat transfer and a pulverization step and a dried bacterial cell powder produced by the method are provided.
Pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus
The invention belongs to the field of chemical analysis and detection, and specifically relates to a pretreatment method for LC-MS detecting metabolomics of Aspergillus flavus. The method includes: culturing a strain of Aspergillus flavus; quenching the Aspergillus flavus; disrupting the cell membrane of Aspergillus flavus, and extracting a metabolome. The invention adopts a cold glycerol buffer solution combined with a rapid filtration method for quenching, and a MeOH/DCM/ACN/EA/HCOOH mixture is used as an metabolome extract, thereby achieving the object of efficiently extracting different polar compounds, and metabolome compound coverage is high; pretreatment of the cell metabolomics of Aspergillus flavus by the method of the invention can ensure the repeatability and stability of the metabolomics analysis method and reduce the false positive of the test results.
ELIMINATION OF EXOTIC PATHOGENS
The present invention includes a mold neutralization system and process based on a hyper-excited mixture composed of a photoexcited carrier constituent within a physiologically inert solution. The efficacy of the system and process can be readily and quickly determined by physiological examination of the organism receiving the system and process.
Method for concentrating a cell suspension comprising a mucilaginous biomass of oleaginous yeasts
A method for concentrating, in order to favour the subsequent extraction process of intracellular lipids, a cell suspension containing a biomass of oleaginous yeasts fermented in fermentation broth under conditions that allow the intracellular accumulation of lipids, where the biomass contains significant quantities of mucilaginous material. The method includes: a) cultivating the oleaginous yeasts in a fermentation broth to obtain a cell suspension containing the mucilaginous biomass; b) subjecting the cell suspension obtained from a) to heat treatment, at a temperature between 95 C. and 120 C. and to acid treatment, to obtain a treated cell suspension containing the mucilaginous biomass containing intact oleaginous yeast cells; and c) concentrating the treated cell suspension obtained from b), by removing at least part of the fermentation broth to obtain a concentrated cell suspension.
COMPOSITE PARTICLES, COMPOSITE PARTICLES FOR FORMING LIQUID-ENCAPSULATING PARTICLES, LIQUID-ENCAPSULATING PARTICLES, METHOD FOR PRODUCING LIQUID-ENCAPSULATING PARTICLES, BIOCATALYST-CONTAINING MATERIAL, BIOCATALYST-CONTAINING MATERIAL PRODUCING APPARATUS, AND BIOCATALYST-CONTAINING MATERIAL PRODUCING METHOD
Provided are composite particles including hydrophobic solid particle A and hydrophobic solid particle B over surface of hydrophobic solid particle A, wherein contact angle CAa of hydrophobic solid particle A with water is 110 degreesCAa180 degrees, contact angle CAb of hydrophobic solid particle B with water is 110 degreesCAb180 degrees, ratio (d50a/d50b) of number average particle diameter d50a of hydrophobic solid particle A to number average particle diameter d50b of hydrophobic solid particle B is 10(d50a/d50b)100, and coating ratio CR of composite particles expressed by Formula 1 is 50%CR500%,