A method of isolating probiotics of human intestine, including preparing bases for culturing liquid and solid mucoproteins; and filtering, which includes collecting human feces, planting the human feces in the base for culturing liquid mucoprotein, culturing the human feces to obtain microbial liquid, using the microbial liquid as template, pouring the microbial liquid into tubes, subjecting the tubes to a PCR test, selecting the tubes tested positive in the PCR test, pouring the microbial liquid in the positive tubes into the base for culturing solid mucoprotein, culturing the microbial liquid to obtain first colonies, planting the colonies in a chocolate tablet, culturing the first colonies to obtain second colonies, planting the second colonies in a Columbia blood tablet, purifying the second colonies to obtain third colonies, using 16sRNA common primers to identify the third colonies in the PCR, and isolating probiotics.

Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.

Devices and methods for isolating and characterizing microbial cells from an environment are provided. The devices integrate sub-micron constrictions in a nanofluidic device with a standard microtiter plate format to facilitate the high throughput isolation, culturing, analysis, and screening of bacteria and other microbial cells in natural and man-made environments, particularly environments containing microbes adhered on particulate matter.

Devices and methods for isolating and characterizing microbial cells from an environment are provided. The devices integrate sub-micron constrictions in a nanofluidic device with a standard microtiter plate format to facilitate the high throughput isolation, culturing, analysis, and screening of bacteria and other microbial cells in natural and man-made environments, particularly environments containing microbes adhered on particulate matter.

A method of treating a biological sample, preferably a sample of blood or bodily fluids likely to contain one or more species of interest, and including a step of decomplexification by acoustophoresis (as well as associated systems, devices, substrates and connection devices).

Described is a method for the culture of microalgae, comprising: providing a consortium of at least two living species of microalgae; culturing under illumination the consortium in a controllable bioreactor and under non-sterile aqueous culture conditions; and controlling the culture conditions for affecting at least one of the following output: (i) flocculation and/or settling of said consortium of microalgae; and (ii) adhesion of the microalgae to surfaces of the bioreactor; wherein said culture conditions are controlled to promote (i) and/or to minimize (ii), without adversely affecting growth of the consortium of microalgae. It is also possible to control the culture conditions for affecting iii) the protein, carbohydrate, and/or fat content of the said microalgae consortium. A system for carrying out the method is also described.

The invention is directed to a method for recovering at least one product from a fermentation broth. The invention relates to the use of a vacuum distillation vessel to recover products, such as ethanol, from a fermentation broth, where the fermentation broth comprises viable microbial biomass, and where the recovery of the product is completed in such a manner to ensure the viability of the microbial biomass. The invention provides for product recovery at an effective rate so as to prevent the accumulation of product in the fermentation broth. To ensure the viability of the microbial biomass, the invention is designed to reduce the amount of stress on the microbial biomass. By ensuring the viability of the microbial biomass, the microbial biomass may be recycled and reused in the fermentation process, which may result in an increased efficiency of the fermentation process.

The invention is directed to a method for recovering at least one product from a fermentation broth. The invention relates to the use of a vacuum distillation vessel to recover products, such as ethanol, from a fermentation broth, where the fermentation broth comprises viable microbial biomass, and where the recovery of the product is completed in such a manner to ensure the viability of the microbial biomass. The invention provides for product recovery at an effective rate so as to prevent the accumulation of product in the fermentation broth. To ensure the viability of the microbial biomass, the invention is designed to reduce the amount of stress on the microbial biomass. By ensuring the viability of the microbial biomass, the microbial biomass may be recycled and reused in the fermentation process, which may result in an increased efficiency of the fermentation process.

Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

The present invention relates to a process for obtaining a biomass composition of a bacterium strain with bactericidal activity which inhibits or kills various pathogenic bacteria.