A device and method for separating and recovering intestinal contents, comprising removing the impurities from the intestinal contents by means of coarse filtration and cross-flow fine filtration, followed by obtaining a concentrate by microporous membrane cross-flow filter, and collecting the target contents. The microporous membrane has a spongy porous structure, and can rapidly and effectively achieve the separation and recovery of flora from the intestinal contents by cooperation with cross-flow filtration. The separated and recovered dry flora comprises at least 10-20% of the initial wet intestinal contents, and comprises at least 10.sup.11/1 g dry flora.

A device and method for separating and recovering intestinal contents, comprising removing the impurities from the intestinal contents by means of coarse filtration and cross-flow fine filtration, followed by obtaining a concentrate by microporous membrane cross-flow filter, and collecting the target contents. The microporous membrane has a spongy porous structure, and can rapidly and effectively achieve the separation and recovery of flora from the intestinal contents by cooperation with cross-flow filtration. The separated and recovered dry flora comprises at least 10-20% of the initial wet intestinal contents, and comprises at least 10.sup.11/1 g dry flora.

A probiotic composition is provided comprising Bacillus amyloliquefaciens strain H57 bacteria that confers health and/or nutritional benefits, including methods of producing and using such a composition. Also provided is a method of treating a 5 probiotic-responsive disease, disorder and condition in an animal and a method of modulating the gastrointestinal flora of an animal, both including administering to said animal a probiotic composition comprising Bacillus amyloliquefaciens strain H57 bacteria.

A probiotic composition is provided comprising Bacillus amyloliquefaciens strain H57 bacteria that confers health and/or nutritional benefits, including methods of producing and using such a composition. Also provided is a method of treating a 5 probiotic-responsive disease, disorder and condition in an animal and a method of modulating the gastrointestinal flora of an animal, both including administering to said animal a probiotic composition comprising Bacillus amyloliquefaciens strain H57 bacteria.

Separation of particles or droplets from a host fluid may be achieved using a transducer and/or reflector that is a thin, non-planar structure. The thin non-planar structure improves operation of an acoustic standing wave generated by an acoustic transducer. The structure may operate as a pressure release boundary and may be constructed as plastic film.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.

A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.

Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.