The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or bio-transformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.

The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or bio-transformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.

An improvement is described for the processing of biological material in a continuous stream by the application of radiant energy taken from the wavelengths from infrared to ultraviolet, and its absorption by a feedstock in a workspace of featuring controlled turbulence created by one or more counter-rotating disk impellers. The absorbed energy and the controlled turbulence patterns create a continuous process of productive change in a feed into the reactor, with separated light and heavy product output streams flowing both inward and outward from the axis in radial counterflow. The basic mechanism of processing can be applied to a wide range of feedstocks, from the promotion of the growth of algae to make biofuel or other forms of aquaculture, to a use in the controlled combustion of organic material to make biochar.

The present invention relates to methods of improving cell lysis procedures and yields of intracellular biomolecules extracted from biomass. The methods comprise storing the microbial cells in ultra-low temperature (ULT) conditions for at least 10 minutes prior to lysing the cells.

The present invention relates to methods of improving cell lysis procedures and yields of intracellular biomolecules extracted from biomass. The methods comprise storing the microbial cells in ultra-low temperature (ULT) conditions for at least 10 minutes prior to lysing the cells.

A process carried out for lysing biological species present in an aqueous solution and for recovering a biological material resulting from the lysis of the biological species, the process being carried out by using a system which includes a vessel delimiting a volume, a first electrode and a second electrode each including an end placed in the volume formed by the vessel, a voltage-pulse-generating circuit. The process includes applying at least one voltage pulse between the two electrodes with the voltage-pulse-generating circuit connected to the two electrodes, so as to bring about the creation of an electric arc between the end of the first electrode and the end of the second electrode and the generation of at least one shockwave in the solution.

The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×10.sup.11 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×10.sup.11 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

Magnetic beads having cell components of interest are translated between a sequence of processing wells in a tray without need for pipetting. The circular tray contains one or more sequences of wells each interconnected by a respective channel. The tray is rotated about a central axis and a magnet, an agitator, and a heater provided external to the tray enable magnetic bead translation, mixing, and incubation, respectively. The magnet proximate a well forms a cluster of beads. Manipulation of the tray in rotation and elevation results in translation of the cluster from one well, through a channel, and into an adjacent well. The well containing a cluster may be rotationally positioned in front of the agitator, the agitator extended into contact with the well, followed by mechanical agitation. The heater, disposed beneath the tray, may accept a well lowered thereto for selective heating.

Magnetic beads having cell components of interest are translated between a sequence of processing wells in a tray without need for pipetting. The circular tray contains one or more sequences of wells each interconnected by a respective channel. The tray is rotated about a central axis and a magnet, an agitator, and a heater provided external to the tray enable magnetic bead translation, mixing, and incubation, respectively. The magnet proximate a well forms a cluster of beads. Manipulation of the tray in rotation and elevation results in translation of the cluster from one well, through a channel, and into an adjacent well. The well containing a cluster may be rotationally positioned in front of the agitator, the agitator extended into contact with the well, followed by mechanical agitation. The heater, disposed beneath the tray, may accept a well lowered thereto for selective heating.