The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

A method for providing a SCP product from a biomass material is shown wherein the SCP product includes a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids in the biomass material, the method including: (i) providing the biomass material; (ii) subjecting the biomass material to a process of cell disruption, providing a disrupted biomass material; (iii) applying the disrupted biomass material to a first separation process resulting in a first retentate comprising proteins and/or cell debris, and a first permeate comprising nucleic acids; (iv) subjecting the first permeate to a second treatment separating the nucleic acids from vitamins, minerals and/or amino acids; (v) optionally, combining the first retentate obtained in step (iii) with the vitamins, minerals and/or amino acids obtained in step (iv), providing the SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids.

A method for providing a SCP product from a biomass material is shown wherein the SCP product includes a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids in the biomass material, the method including: (i) providing the biomass material; (ii) subjecting the biomass material to a process of cell disruption, providing a disrupted biomass material; (iii) applying the disrupted biomass material to a first separation process resulting in a first retentate comprising proteins and/or cell debris, and a first permeate comprising nucleic acids; (iv) subjecting the first permeate to a second treatment separating the nucleic acids from vitamins, minerals and/or amino acids; (v) optionally, combining the first retentate obtained in step (iii) with the vitamins, minerals and/or amino acids obtained in step (iv), providing the SCP product comprising a reduced amount of nucleic acids relative to the naturally occurring amount of nucleic acids.

Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

The present disclosure is generally directed to an anucleated cell-based platforms for encapsulation and delivery of agricultural compounds. Disclosed herein are compositions for the stable and targeted delivery of agricultural compounds within achromosomal and/or anucleated cells. The present disclosure also provides methods of improving encapsulation and retention of agricultural compounds in achromosomal and/or anucleated cells.

Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.