The invention discloses a fermentation method for production of fucoxanthin by Nitzschia laevis, including the following steps of: step A, preparation of inocula; step B, fermentation culture: inoculating of Nitzschia laevis according to a certain volume ratio to reaction kettle containing sterile fermentation medium for aeration fermentation, preparing fucoxanthin fermentation broth through culture mean of fed-batch nutrient components; step C, obtaining high fucoxanthin induction culture solution by aeration induction culture under irradiation of monochromatic light or mixed light; extracting fucoxanthin from high fucoxanthin induction culture solution. The invention optimized fermentation condition by fed-batch nutrient components during aeration culture of alga Nitzschia laevis, thereby significantly increasing the cell density of Nitzschia laevis in sterile fermentation broth, and then treating high density fucoxanthin induction culture solution of Nitzschia laevis by using light treatment, inducing the accumulation of fucoxanthin, thereby further increasing productivity of fucoxanthin produced by fermentation.

The invention discloses a fermentation method for production of fucoxanthin by Nitzschia laevis, including the following steps of: step A, preparation of inocula; step B, fermentation culture: inoculating of Nitzschia laevis according to a certain volume ratio to reaction kettle containing sterile fermentation medium for aeration fermentation, preparing fucoxanthin fermentation broth through culture mean of fed-batch nutrient components; step C, obtaining high fucoxanthin induction culture solution by aeration induction culture under irradiation of monochromatic light or mixed light; extracting fucoxanthin from high fucoxanthin induction culture solution. The invention optimized fermentation condition by fed-batch nutrient components during aeration culture of alga Nitzschia laevis, thereby significantly increasing the cell density of Nitzschia laevis in sterile fermentation broth, and then treating high density fucoxanthin induction culture solution of Nitzschia laevis by using light treatment, inducing the accumulation of fucoxanthin, thereby further increasing productivity of fucoxanthin produced by fermentation.

The present invention provides a process for the microbiological production of hydrogen from a hydrocarbon-rich deposit, said process comprising the step of modifying the composition of the deposit by the introduction into the deposit of at least one non-native hydrogen producing microorganism selected positively to diversify the microbiological abundance of hydrogen-producing microorganisms in the deposit and for the preferential production of hydrogen over methane.

In one embodiment, an algae cultivation system includes a basin that contains a liquid and a photobioreactor at least partially immersed in the liquid of the basin, the photobioreactor comprising a closed container including multiple panels that together define an interior space in which algae can be cultivated, at least one of the panels being transparent, the photobioreactor further comprising an inflatable float associated with the container that can be filled with a gas to change one or both of the position and orientation of the container within the liquid.

In one embodiment, an algae cultivation system includes a basin that contains a liquid and a photobioreactor at least partially immersed in the liquid of the basin, the photobioreactor comprising a closed container including multiple panels that together define an interior space in which algae can be cultivated, at least one of the panels being transparent, the photobioreactor further comprising an inflatable float associated with the container that can be filled with a gas to change one or both of the position and orientation of the container within the liquid.

Disclosed herein are novel methods for hydrolyzing eggshell membrane (ESM). In one embodiment, the method includes cultivating thermophilic bacteria in a solution containing 1-10% (wt %) ESM to decompose the ESM into the ESM hydrolysate; wherein, the thermophilic bacteria grow on the ESM as their sole source of nutrient. In another embodiment, the method includes treating ESM with a keratinase in the presence of a reducing agent at a condition sufficient to produce the ESM hydrolysate, in which the keratinase, the reducing agent, and the ESM are present in a weight ratio of 1:120:600. The thus produced ESM hydrolysate is enriched in essential amino acids, collagen, peptides and glycosaminoglycans.

Disclosed herein are novel methods for hydrolyzing eggshell membrane (ESM). In one embodiment, the method includes cultivating thermophilic bacteria in a solution containing 1-10% (wt %) ESM to decompose the ESM into the ESM hydrolysate; wherein, the thermophilic bacteria grow on the ESM as their sole source of nutrient. In another embodiment, the method includes treating ESM with a keratinase in the presence of a reducing agent at a condition sufficient to produce the ESM hydrolysate, in which the keratinase, the reducing agent, and the ESM are present in a weight ratio of 1:120:600. The thus produced ESM hydrolysate is enriched in essential amino acids, collagen, peptides and glycosaminoglycans.

A process of growing a phototrophic biomass in a reaction zone, including a reaction mixture that is operative for effecting photosynthesis upon exposure to photosynthetically active light radiation, is provided. The reaction mixture includes phototrophic biomass that is operative for growth within the reaction zone. In one aspect, the carbon dioxide supply is modulated in response to detected process parameters. In another aspect, inputs to the reaction zone are modulated based on changes to the carbon dioxide supply. In another aspect, dilution of the carbon dioxide-comprising supply is effected. In another aspect, pressure of the carbon dioxide-comprising supply is increased. In another aspect, water is condensed from the carbon dioxide-comprising supply and recovered for re-use. In another aspect, the produced phototrophic biomass is harvested at a rate which approximates a predetermined mass growth rate of the phototrophic biomass.

A process of growing a phototrophic biomass in a reaction zone, including a reaction mixture that is operative for effecting photosynthesis upon exposure to photosynthetically active light radiation, is provided. The reaction mixture includes phototrophic biomass that is operative for growth within the reaction zone. In one aspect, the carbon dioxide supply is modulated in response to detected process parameters. In another aspect, inputs to the reaction zone are modulated based on changes to the carbon dioxide supply. In another aspect, dilution of the carbon dioxide-comprising supply is effected. In another aspect, pressure of the carbon dioxide-comprising supply is increased. In another aspect, water is condensed from the carbon dioxide-comprising supply and recovered for re-use. In another aspect, the produced phototrophic biomass is harvested at a rate which approximates a predetermined mass growth rate of the phototrophic biomass.

The present invention relates to mutants of lactic acid bacteria which are resistant to the antibiotic ampicillin and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant to ampicillin.