A system and method for making a liquid chemistry treated biopolymer-based fungal mat is described. The method comprises the steps of harvesting a plurality of fresh mycelium material and marking them for identification, then weighing and recording the initial mass of each of the plurality of mycelium material is carried out. A liquid chemical solution using solvent: chemical ratios from 0:100 to 100:0 is prepared. Next, decanting the liquid chemical solution into a vacuum tumbler drum distributed with the mycelium material. Applying vacuum and rotating the vacuum tumbler drum to ensure thorough mixing and refreshing of the liquid chemical solution at the mycelium surface. Vacuuming and rotating the vacuum tumbler drum is repeated and the at least one fungal mat formed is removed from the vacuum tumbler drum. Finally, draining away surface moisture and air drying the at least one fungal mat.

The present invention relates to a method for producing a composite fermentation substrate comprising a) Mixing of at least one thermoplastic with a starch containing, organic, granular or powdery, non-liquifiable growth medium; and b) Melt extruding the mixture obtained from a) into a desired shape. Furthermore, the invention relates to composite substrates produced according to the present method and a method for producing a microorganism, comprising (a) Providing a composite substrate according to the invention, (b) Inoculating the composite substrate with a microorganism to be cultivated; and (c) Incubating the composite substrate obtained from step (b) under controlled conditions.

The present invention relates to a method for producing a composite fermentation substrate comprising a) Mixing of at least one thermoplastic with a starch containing, organic, granular or powdery, non-liquifiable growth medium; and b) Melt extruding the mixture obtained from a) into a desired shape. Furthermore, the invention relates to composite substrates produced according to the present method and a method for producing a microorganism, comprising (a) Providing a composite substrate according to the invention, (b) Inoculating the composite substrate with a microorganism to be cultivated; and (c) Incubating the composite substrate obtained from step (b) under controlled conditions.

A method for producing a protein is provided. An objective protein is produced by culturing in a culture medium Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a Pep4 protein is reduced.

The present disclosure relates to the technical field of Gastrodia elata planting, in particular to an organic planting method of G. elata, including the process of mycelia colonized material preparation: cutting a branch of Quercus acutissima into bars; cutting out fish scale openings on the bars to obtain bars with the fish scale openings; successively soaking the bars with the fish scale openings in boiling water and a growth-promoting solution to obtain bars after growth promotion treatment; and putting Amillariella mellea spawns and an inoculation substrate into each fish scale opening of the bars after the growth promotion treatment to obtain a mycelia colonized material. The growth-promoting solution and the inoculation substrate are prepared by treatment of fresh G. elata stalks with Bacillus pumilus and hot-dip extraction treatment in ethanol.

The present disclosure relates to the technical field of Gastrodia elata planting, in particular to an organic planting method of G. elata, including the process of mycelia colonized material preparation: cutting a branch of Quercus acutissima into bars; cutting out fish scale openings on the bars to obtain bars with the fish scale openings; successively soaking the bars with the fish scale openings in boiling water and a growth-promoting solution to obtain bars after growth promotion treatment; and putting Amillariella mellea spawns and an inoculation substrate into each fish scale opening of the bars after the growth promotion treatment to obtain a mycelia colonized material. The growth-promoting solution and the inoculation substrate are prepared by treatment of fresh G. elata stalks with Bacillus pumilus and hot-dip extraction treatment in ethanol.

A method for preparing a fermented food by using a Rhizopus microsporus strain is provided. The method includes the following steps: providing an isolated and purified Rhizopus microsporus strain, and its deposit number is DSM 34400; and inoculating the isolated and purified Rhizopus microsporus strain to a substrate for fermentation to form a fermented food. The substrate includes a legume, a processing residue of a legume, or a combination thereof.

A composition for remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

A composition for remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

In a method of controlling a weed in a soil, an effective amount of a solid composition is applied on the soil. The solid composition includes fungal dormant sclerotium, a carbon source nutrient, and a nitrogen source nutrient. The solid composition is a dried solid composition. The solid composition may weed out target plants, including the burcucumber which is designated as an ecologically damaging plant and suppress soil-borne diseases like Sclerotinia dollar spot disease. By using the solid composition, burcucumber and Sclerotinia dollar spot disease can effectively be controlled, and it is possible to make significant gains in solving problems with biological products containing microorganisms in stages of production, storage, distribution, and usage process.