The present invention relates to Candida strains comprising a mutation or deletion in the first and/or second XYL2 allele which can be used for producing one or more sugar alcohols from a lignocellulosic feedstock. The preferred sugar alcohol is xylitol.

The present invention relates to Candida strains comprising a mutation or deletion in the first and/or second XYL2 allele which can be used for producing one or more sugar alcohols from a lignocellulosic feedstock. The preferred sugar alcohol is xylitol.

The disclosure provides compositions and methods for growing programmable enzyme-functionalized and sense-and-response bacterial cellulose living materials with engineered microbial co-cultures.

The disclosure provides compositions and methods for growing programmable enzyme-functionalized and sense-and-response bacterial cellulose living materials with engineered microbial co-cultures.

A method for preparing a universal microbial medium by eutectic system-based cellulose liquefaction. The method includes: 1) mixing ionic liquid and cellulose, where the ionic liquid is preheated; 2) mixing a mixed solution I obtained in step 1) and distilled water; 3) filtering a mixed solution II obtained in step 2); and 4) diluting a filtrate obtained in step 3) with the distilled water to obtain the carbon source. A medium prepared from the carbon source can be used for culturing various microorganisms, with a desirable growth state of the microorganisms.

A method for preparing a universal microbial medium by eutectic system-based cellulose liquefaction. The method includes: 1) mixing ionic liquid and cellulose, where the ionic liquid is preheated; 2) mixing a mixed solution I obtained in step 1) and distilled water; 3) filtering a mixed solution II obtained in step 2); and 4) diluting a filtrate obtained in step 3) with the distilled water to obtain the carbon source. A medium prepared from the carbon source can be used for culturing various microorganisms, with a desirable growth state of the microorganisms.

To culture cells in a suspended state and easily recover the cultured cells. An additive for suspension culture according to an embodiment is a medium additive which is added to a medium for culturing cells in suspension, the medium additive containing a cellulose oligomer. A medium composition according to an embodiment is a medium composition capable of culturing cells in suspension, the medium composition containing a cellulose oligomer. A cell culture method according to an embodiment includes culturing cells in the medium composition.

To culture cells in a suspended state and easily recover the cultured cells. An additive for suspension culture according to an embodiment is a medium additive which is added to a medium for culturing cells in suspension, the medium additive containing a cellulose oligomer. A medium composition according to an embodiment is a medium composition capable of culturing cells in suspension, the medium composition containing a cellulose oligomer. A cell culture method according to an embodiment includes culturing cells in the medium composition.

Provided are aqueous fermentation feedstocks comprising glucose monomers at a concentration of less than 50 gram/Liter (g/L) of the total feedstock, water-soluble dextrose oligomers at a concentration in a range between 50 g/L and 300 g/L of the total feedstock; and water. Further provided are methods of production thereof and uses thereof in the production of single cell protein and/or ethanol.

Provided are aqueous fermentation feedstocks comprising glucose monomers at a concentration of less than 50 gram/Liter (g/L) of the total feedstock, water-soluble dextrose oligomers at a concentration in a range between 50 g/L and 300 g/L of the total feedstock; and water. Further provided are methods of production thereof and uses thereof in the production of single cell protein and/or ethanol.