An oil sludge treatment system includes: a reduced-pressure fermentation dryer; a filtration apparatus; and a cleaner. The reduced-pressure fermentation dryer is configured to: store oil sludge in an airtight container; heat and stir the oil sludge under reduced pressure so that a temperature of the oil sludge is within a predetermined temperature range; decompose organic matter contained in the oil sludge using microorganisms while evaporating water; and obtain volume-reduced dried product. The filtration apparatus filters oil from the dried product. The cleaner cleans, by steam, the dried product from which the oil has been filtered.

An oil sludge treatment system includes: a reduced-pressure fermentation dryer; a filtration apparatus; and a cleaner. The reduced-pressure fermentation dryer is configured to: store oil sludge in an airtight container; heat and stir the oil sludge under reduced pressure so that a temperature of the oil sludge is within a predetermined temperature range; decompose organic matter contained in the oil sludge using microorganisms while evaporating water; and obtain volume-reduced dried product. The filtration apparatus filters oil from the dried product. The cleaner cleans, by steam, the dried product from which the oil has been filtered.

Poly and perfluoroalkyl substances (PFAs) are extremely resistant to natural degradation. Described herein are compositions, assays, and methods for generating microorganisms capable of accelerating degradation of these products using a directed evolution strategy. A minimal media including the target carbon source of interest, creates selective pressure for microorganisms capable of degradation. Extremely slow growth rates on these alternative carbon sources is nevertheless measureable using automated image capture and processing pipeline allows one to observe very small changes in cell growth over the course of a very long period of time, thereby identifying microorganisms that could not otherwise be identified.

Disclosed herein is a method of microbial enhanced oil recovery by changing microbial motility, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility. This application also provides a method for microbial enhanced oil recovery, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility; and injecting the contact culture mixture of the viable Glycocaulis strain and the alkane-degrading Dietzia strain to an oil well to perform microbial enhanced oil recovery.

Disclosed herein is a method of microbial enhanced oil recovery by changing microbial motility, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility. This application also provides a method for microbial enhanced oil recovery, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility; and injecting the contact culture mixture of the viable Glycocaulis strain and the alkane-degrading Dietzia strain to an oil well to perform microbial enhanced oil recovery.

The present disclosure relates to glycolipid compositions, methods for making glycolipid compositions, and their uses thereof. Glycolipid compositions can be prepared via yeast-mediated catalyzed reaction, and exhibit excellent surfactant properties having high corrosion inhibition performance, good reducing surface tension efficiency. Processes of the present disclosure can provide glycolipid compositions having one or more of: a ratio of lactonic glycolipids to glycolipid acylic esters is from about 1:10 to about 10:1, a molecular weight of from about 400 g/mol to about 10,000 g/mol, a corrosion rate of carbon steel from about 0.5 MPY to about 100 MPY at room temperature and at pH 4-6. Furthermore, aqueous solutions of the glycolipid compositions of the present disclosure can have a surface tension of from about 20 mN/m to about 80 mN/m.

Disclosed herein is a method of microbial enhanced oil recovery by changing microbial motility, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility. This application also provides a method for microbial enhanced oil recovery, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility; and injecting the contact culture mixture of the viable Glycocaulis strain and the alkane-degrading Dietzia strain to an oil well to perform microbial enhanced oil recovery.

Disclosed herein is a method of microbial enhanced oil recovery by changing microbial motility, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility. This application also provides a method for microbial enhanced oil recovery, including: subjecting a viable Glycocaulis strain and the alkane-degrading Dietzia strain to contact culture to enable the alkane-degrading Dietzia strain to have motility; and injecting the contact culture mixture of the viable Glycocaulis strain and the alkane-degrading Dietzia strain to an oil well to perform microbial enhanced oil recovery.

Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.

Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.