The present application relates to systems and methods for transporting biological samples, including a transport medium configured to protect bacterial viability and optimize sample quality, for enhanced diagnostic accuracy. The disclosed systems and methods can include a transport media, a collection tube including a screen and sampling brush, and a transport case. The disclosed systems and methods allow samples to be frozen and thawed while preserving cellular wall integrity during transport or storage, and furthermore can preserve the survival of the bacterial sample, enabling more accurate detection of an infectious agent.

Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. Escherichia coli) or a eukaryote (e.g. Yarrowia lipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.

Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. Escherichia coli) or a eukaryote (e.g. Yarrowia lipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.

Provided herein are metabolically-modified microorganisms that can grow on an organic C1 carbon source.

Provided herein are metabolically-modified microorganisms that can grow on an organic C1 carbon source.

The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes is activated, upregulated or downregulated. Also provided are methods for increasing cellular glycerol uptake and increasing recombinant production of fuels and other chemicals using the recombinant microorganisms of the invention.

The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes is activated, upregulated or downregulated. Also provided are methods for increasing cellular glycerol uptake and increasing recombinant production of fuels and other chemicals using the recombinant microorganisms of the invention.

The present invention generally relates to the biotechnology engineering, and specifically to genetically modified bacteria of the genus Methylobacillus which have improved properties making them particularly useful in large scale methanol fermentations. More specifically, the present invention provides a bacterium of the genus Methylobacillus which has been modified to have a decreased production of exopolysaccharides (EPS) compared to an otherwise identical bacterium that does not carry said modification. The present invention further provides a method for producing a biochemical compound using a genetically modified bacterium of the present invention.

The present invention generally relates to the biotechnology engineering, and specifically to genetically modified bacteria of the genus Methylobacillus which have improved properties making them particularly useful in large scale methanol fermentations. More specifically, the present invention provides a bacterium of the genus Methylobacillus which has been modified to have a decreased production of exopolysaccharides (EPS) compared to an otherwise identical bacterium that does not carry said modification. The present invention further provides a method for producing a biochemical compound using a genetically modified bacterium of the present invention.

The present application provides a process for sequestering carbon dioxide to produce a biomass containing reaction product, the process comprising the steps of: I. contacting a raw carbon dioxide-containing feedstock with an absorption or dissolution medium to form a reagent stream comprising dissolved or absorbed inorganic carbon at least in the form of HCO.sub.3.sup.- and CO.sub.3.sup.2- wherein the HCO.sub.3.sup.- :CO.sub.3.sup.2- molar ratio in the reagent stream is at least about 0.8; II. contacting at least a portion of the reagent stream with a microbial broth in a bioreactor to produce a biomass-containing reaction product; III. separating the biomass-containing reaction product into a biomass product and a liquid stream; and IV. recycling at least a portion of the liquid stream to step i. of the process for use as, or as part of, the absorption or dissolution medium,wherein the pH of the absorption or dissolution medium is controlled to maintain the HCO.sub.3.sup.- :CO.sub.3.sup.2- molar ratio in the reagent stream at least about 0.8.