C12N1/38

Increasing ornithine accumulation to increase high mannose glycoform content of recombinant proteins

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

Increasing ornithine accumulation to increase high mannose glycoform content of recombinant proteins

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

Culture conditions that allow Zymomonas mobilis to assimilate N2 gas as a nitrogen source during bio-ethanol production

Chemically defined culture medium and culture conditions that allow bacteria to assimilate dinitrogen gas (N.sub.2) as a nitrogen source during bio-ethanol production are disclosed herein. Methods of bioethanol production using the chemically defined culture medium and culture conditions are also disclosed.

Culture conditions that allow Zymomonas mobilis to assimilate N2 gas as a nitrogen source during bio-ethanol production

Chemically defined culture medium and culture conditions that allow bacteria to assimilate dinitrogen gas (N.sub.2) as a nitrogen source during bio-ethanol production are disclosed herein. Methods of bioethanol production using the chemically defined culture medium and culture conditions are also disclosed.

Method of cultivating algae

The present description is related to the field of cultivating algae. It introduces a method of cultivating algae by depleting the culture of an inorganic nutrient and exposing the alga to high intensity light to obtain algal cell mass having enriched lipid content and reduced chlorophyll content.

Method of cultivating algae

The present description is related to the field of cultivating algae. It introduces a method of cultivating algae by depleting the culture of an inorganic nutrient and exposing the alga to high intensity light to obtain algal cell mass having enriched lipid content and reduced chlorophyll content.

Method of producing a recombinant protein

The present invention relates to a method of producing a recombinant protein in a host cell comprising adding Polyethyleneimine (PEI) during cell culture. Addition of PEI to the cell culture as a fermentation enhancer can result in reducing the viscosity of the cell culture, and/or increasing the extracellular concentration of the recombinant protein, and/or reducing the duration of cell culture to the point of harvest or protein recovery.

Method of producing a recombinant protein

The present invention relates to a method of producing a recombinant protein in a host cell comprising adding Polyethyleneimine (PEI) during cell culture. Addition of PEI to the cell culture as a fermentation enhancer can result in reducing the viscosity of the cell culture, and/or increasing the extracellular concentration of the recombinant protein, and/or reducing the duration of cell culture to the point of harvest or protein recovery.

Methods for increasing mannose content of recombinant proteins

The present invention relates to methods of modulating the mannose content of recombinant proteins.

Methods for increasing mannose content of recombinant proteins

The present invention relates to methods of modulating the mannose content of recombinant proteins.